Sterilization and Disinfection Flashcards

1
Q

What is sterilization

A

Destroys all Microbial Life including spores.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

What is disinfection (usually chemical)

A

Destroys pathogens, other microbes/ Spores may survive

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

When would we use sterilization

A

Processing scalpel blades/ dental equipment to be used in surgery OR anything that requires touching a sterile movement

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

When would we use disinfection?

A

Cleansing work surfaces with bleach solution or disinfectant wipe after each shift

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

What is antisepsis

A

Cleansing the skin before giving an injection

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

What is sanitation

A

Used dishes are washed with detergent and hot water followed by hot water rinse

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

What is cleaning

A

Washing used instruments with detergent and water before further processing

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

What is cidal

A

death to all. A couple of examples are germicide, bactericide, and fungicide

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

What is static

A

stopping microbial growth. Examples include Bacteriostatic and fungistatic

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

What is a SUD

A

Single use device. Usually syringes

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

What are three types of sterilization methods

A

Autoclave
Incineration
ethylene oxide (ETO)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

What is an Autoclave

A

Sterilization machine. Done under pressure; “moist heat”

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

What is the temperature and the time needed to sterilize in a autoclave

A

121 degrees C; 15 minutes

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

What is the loading process of an autoclave

A
  1. Load items onto perforated tray
  2. Load Loosely
  3. Dry goods on edge; lids ajar, jars, tubes»> on sides
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

What is the testing process for an autoclave

A
  1. spore strips are placed in autoclave (center and bottom)
  2. Place strings in culture medium after autoclaving»> incubate&raquo_space;» check for growth
  3. growth = spores not killed = autoclave not working properly
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

What happens if an autoclave package gets wet

A

It is no longer sterile

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

What is Incineration

A

sterilization method which is done offsite where terminal decontamination for biological waste (used needles) happen. We burn at a very high temp

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

What is Ethylene Oxide

A

Sterilization method that kills all microbes including spores.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

When would we use ethylene oxide

A

Heat sensitive items (plastics)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

Where would we commonly find Ethylene Oxide

A

Sterile processing dept.

21
Q

Is ethylene safe for human use?

A

It is actually toxic to human tissue

22
Q

What is Spaulding’s classification of items requiring disinfection/ sterilization

A

Critical
semi critical
non critical

23
Q

What does Spaulding classify as critical

A

into sterile area of the body; must be sterile (surgical equipment)

24
Q

What does Spaulding classify as semi- critical

A

into/touching area of body that is not sterile; no pathogen, but doesn’t need to be sterile (tongue depressor into mouth)

25
What does Spaulding classify as non-critical
Not into body, but may touch body surface; ok to have microbes, but not if can be inhaled or cause infection (gloves)
26
Resistance of Microbes disinfection least to most resistant
1. Vegetative bacteria (easiest to destroy) 2. Liquid Viruses ( except hep B) 3. Fungi 4. Non-Lipid viruses 5. Tubercle bacilli 6. Bacterial spores
27
What are the different levels of disinfectants
low intermediate high level
28
What can low level disinfectants do
Kill vegetative bacteria and lipid viruses
29
What can a intermediate level disinfectant do
kill everything except non-lipid virus and spores
30
What can a high level disinfectant do
everything
31
What are examples of low level disinfectants
Detergents and soap
32
What can detergents and soap do
reduce surface tension so liquid can spread into nooks
33
What is a example of a detergent and soap
Quaternary ammonium compounds
34
What are advantages of Quaternary ammonium compounds (detergents)
economical, kills gram + bacteria
35
What are disadvantages of Quaternary ammonium compounds
Rods may grow
36
What are intermediate levels of disinfection/ sterilization
Alcohols (ethanol and isopropyl) used for antisepsis, disinfect thermometers
37
What is the optimum concentration for cleaning alcohols
70%
38
What is an example of a intermediate level disinfection/ sterilization
Phenolic compounds
39
What are examples of Phenolic compounds
Hexachlorophene | Chlorhexidine
40
What is the shelf life of chlorine containing agents
24 hour
41
What are advantages of bleach- chorine detergent
cheap, available, kills vegetative bacteria (rapid), kills tubercle bacilli and viruses
42
What are disadvantages of bleach- chlorine detergents
not good for metal, plastic, rubbers; irritates skin/eyes, v high concentration kills spores
43
What is a example of a strong detergent
when dilution is 1 in 10
44
What are examples of High grade chemical disinfection/ sterilization
Glutaraldehyde and Peroxygen
45
What is the shelf life for glutaraldehyde
limited shelf life after activation
46
What are advantages of glutaraldehyde
broad spectrum, not inactivated by organic materials
47
What are disadvantages of glutaraldehyde
money, toxic, irritating fumes
48
What are examples of mechanical disinfection
Pasteurization and filtration
49
What are misc. methods of microbial control
UV light radiation, Ionizing radiation, moist heat (boiling)