Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

Microbiology > Sterilization > Flashcards

Sterilization Flashcards

You may prefer our related Brainscape-certified flashcards:
Biology 101 flashcards
1
Q

Biologic Agents that are acquired by ingestion, percutaneous exposure

A

Biosafety level 2

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Chamber filled with hot steam under pressure

A

Autoclave

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Removal or destruction of all forms of life

A

Sterilization

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

Most commonly used method for the removal of microorganisms

A

Heat

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

Biological indicator of autoclave

A

Bacillus stearothermophilus

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

Temperature, psi for decontaminating medical waste using autoclave

A

132C 15psi for 30mins

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Destroys vegetative or non sporulating bacteria

A

Boiling

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

Instrument used in tyndallization

A

Arnold’s sterilizer or free flowing steam

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

Fastest and simplest method of sterilization

A

Autoclave

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

Temperature and time of exposure for boiling

A

100C for 10-15mins

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

Temperature and time of exposure for tyndallization

A

100C for 30mins in 3 consecutive days

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

Used to sterilize protein rich media. Such as LJ

A

Inspissation

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

Used to sterilize milk, dairy products and alcoholic beverages

A

Pasteurization or partial sterilization

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

Temperature, psi and minutes to sterilize media, liquids, glass pipets and instruments for assay using autoclave

A

121C, 15psi for 15 mins

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

Temperature and time of exposure for inspissation

A

70-80C for 2hrs in 3 consecutive days

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

This method cannot eliminate bacterial endospores

A

Pasteurization

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

Treatment at this temperature reduces spoilage if food without affecting its taste

A

Low temperature holding or

Batch method

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

Utilized for sterilization of glasswares, oil products and powders

A

Dry heat procedure-oven

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
23
Q

Destroys milk pathogens

A

Batch method

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
24
Q

Temperature and time of exposure for batch method

A

63C for 30mins

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
25
Q

Temperature and time of exposure for flash pasteurization

A

72C for 15sec

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
26
Q

Cooled very quickly in a vacuum chamber

A

Ultra high temperature

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
27
Q

Milk can be stored at room temperature for two months without affecting its flavor

A

Ultra high temperature

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
28
Q

Principle of inspissation

A

Thickening of media through evaporation

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
29
Q

Temperature and time exposure for ultra high temperature

A

140C for 3sec

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
30
Q

Not applicable for heat sensitive material

A

Dry heat procedure

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
31
Q

Biological indicator of Dry heat procedure

A

Bacillus subtilis var niger

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
32
Q

Direct heating

A

Flaming

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
33
Q

Temperature and time of exposure for oven

A

160-170C for 1.5-2hrs

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
34
Q

Sterilization of heat sensitive solution

A

Filtration

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
35
Q

Kills microorganisms by denaturation of proteins

A

Dry heat procedure

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
36
Q

Most common method of treating infectious waste and infected laboratory animals

A

Incineration

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
37
Q

Principle of incineration

A

Burning materials into ashes in 300-400C

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
38
Q

Potential biologic agents for aerosol transmission, lethal pathogens

A

Biosafety level 3

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
39
Q

Used to control the spread of communicable diseases

A

Cremation

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
40
Q

Refers to the lowest temperature at which a suspension of bacteria is killed in 10mins

A

Thermal death point

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
43
Q

Shortest period of time needed to kill a suspension of bacteria at a prescribe temperature and under specific conditions

A

Therrmal death time

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
44
Q

The time in a minute to reduce the bacterial population or spores by 90% at a specified temperature

A

Decimal reduction time

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
45
Q

Quick heating then immediate cooling

A

High temperature short time
Or
Flash pasteurization

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
46
Q

Two types of filter

A

Depth and membrane filters

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
47
Q

Destroys bacteria by changes in proteins and decreased chemical reactions

A

Lyophilization

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
48
Q

Method of choice for sterilization of antibiotic solutions, toxic chemicals, radioisotopes, vaccines

A

Filtration

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
49
Q

Consists of fibrous or granular material

A

Depth filters

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
50
Q

Easily transmitted and highly infectious agent of bioterrorism

A

Category A

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
51
Q

These are porous membranes almost 0.1mm thick. Composed of cellulose acetate or polycarbonate

A

Membrane or circular filter

52
Q

Uses cellulose acetate or cellulose nitrate with a vacuum

A

Liquid filtration

53
Q

Uses HEPA filer

A

Air filter

54
Q

Used to sterilized biohazardous trash and heat stable objects

A

Autoclave

55
Q

Remove organisms larger than 0.3um from isolation rooms, OR and biological safety cabinet

A

High efficiency particulate air (HEPA) filter

56
Q

Destroys bacteria by disruption of metabolism

A

Dessication

57
Q

Destroys microorganisms by coagulation of enzymes and structural proteins and degradation of nucleic acid

A

Moist heat procedure

58
Q

Bacteriostatic.

Reduces the rate of metabolism

A

Low or cold temperatures

60
Q

Bacterias that can withstand dryness

A

N. gonorrheae (1hr)
MTB- several months
Bacillus and clostridium 10yrs

61
Q

Pore size of membrane filters that remove most bacteria as well as fungi but not viruses

A

0.2-0.45um

62
Q

Uses 0.22um membrane filters for parenteral solution

A

Critical sterilizing

63
Q

Membrane filter pore size that used to remove vegetative cells

A

0.22um

64
Q

Important in food microbiology

A

Low or cold temperatures

65
Q

Involves removing water from microbes

A

Dessication

66
Q

Most commonly used BSC in clinical lan

A

Class IIA

67
Q

Most effective method for long term preservation of microbial cultures

A

Lyophilization

68
Q

Used in the form of hypochlorite

A

Chlorine

69
Q

Turning culture into powder

A

Lyophilization

70
Q

Use of high concentrations of salts and sugars in food create a hypertonic environment

A

Osmotic pressure

71
Q

Biological indicator of radiation

A

Bacillus pumilus

72
Q

Causes mutation in DNA and produce peroxidase

A

Ionizing radiation or cold sterilization

73
Q

Treponema pallidum is killed when exposed to

A

2-8C for 72hrs

74
Q

Biologic agents that Include all common agents of infectious diseases

A

Biosafety level 2

75
Q

Applied topically to skin

A

Antiseptic

78
Q

Destroys vegetative cells and endospores of both prokaryotes and eukaryotes

A

Ionizing radiation

80
Q

Examples are B. Subtilis and N. Gruberi

A

Biosafety level 1

81
Q

Also used to “pasteurize” meat products

A

Ionizing radiation

82
Q

Causes damage to cellular DNA by producing thymine dimers

A

Non ionizing radiation

83
Q

Refers to removal, inhibition or killing of microorganisms including potential pathogens by using chemical agents usually on inanimate objects

A

Disinfection

84
Q

Applied to inanimate object

A

Disinfectant

85
Q

Dangerous and exotic biologic agent. Cause life threatening infection

A

Biosafety level 4

86
Q

Should be allowed to evaporate from the surface to which they were applied to achieve maximum effectivity

A

Alcohol

87
Q

Biologic agent that Include those that have no known potential for infecting healthy people

A

Biosafety level 1 agents

88
Q

Bioterrorism agent with moderate morbidity and low mortality

A

Category B

89
Q

When it passes through the cells, it creates free hydrogen and hydroxyl radicals and some peroxidase which in turn cause different intracellular damage

A

Radiation

90
Q

It kills all bacteria in the specimen

A

Bactericidal

91
Q

Hydrolyzes and coagulates proteins

A

Acid and alkaline solution

93
Q

Destroys microorganisms by oxidation mechanism

A

Halogen

94
Q

First widely used antiseptic and disinfectant

A

Phenol

95
Q

For cleaning blood spills

A

5.25% hypochlorite

98
Q

Combination of iodine and neutral polymer

A

Iodophors

99
Q

Betadine must be in contact with an object for how long

A

1-2mins

101
Q

Destroys microorganism by inactivation and precipitation of cell proteins

A

Salts of heavy metals

102
Q

Eye drop solution.

A

AgNO3

103
Q

Bioterrorism agent. Emerging pathogens

A

Category C

104
Q

Widely used as surface active agents

A

Detergent

105
Q

Provides most protection to worker

A

Class III cabinet

106
Q

Bacteria that is resistant to detergent

A

Pseudomonas aeruginosa

107
Q

Sterilization without water

A

Dry heat procedure

108
Q

Sterilizes HEPA filter in BSC

A

Formaldehyde

109
Q

Highest dilution that kills bacteria after a 10mins exposure

A

Phenol coefficient

110
Q

Used to disinfect drinking water

A

Halozone

111
Q

Pose the greatest public health threat

A

Category A

112
Q

Has a rapid killing action but does not penetrate organic material

A

Glutaraldehyde

113
Q

Also known as laminar flow BSC

A

Class II cabinet

114
Q

Biologic agent Used in lab teaching exercises

A

Biosafety level 1

115
Q

Commonly used gas for sterilization

A

Ethylene oxide

116
Q

Inhibits the growth of organism

A

Bacteriostatic

117
Q

Biological indicator of EtO

A

Bacillus subtilis var. globijii

118
Q

More stable than iodine in its pure form

A

Iodophor

119
Q

Active against all vegetative microorganisms and fungal spores

A

Periacetic acid

121
Q

PC result that indicates that the disinfectant is more effective than phenol

A

PC>1

123
Q

Device that encloses a working area to protect workers from aerosol exposure

A

Biological safety cabinet

124
Q

Open fronted type of cabinet with negative pressure

A

Class I cabinet

126
Q

Used to sterilize heart lung machine

A

Ethylene oxide

136
Q

May e used with both liquid and air

A

Filtration

141
Q

Principle f autoclave

A

Steam under pressure

Microbiology (1 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions