Sterilisation 1 Flashcards
2 methods of sterilisation
produce under clean conditions then terminally sterilise outer container- more common
produce and assemble under sterile conditions
bacteria on synthetic materials?
low microbe content
get mostly from us handling them
bacteria on semi-synthetic materials?
low microbe content, come mainly through us handling them
bacteria on natural products?
have their own intrinsic microflora, vary depending on the products nature
what is the primary requirement for bacterial growth
water
TF: air is a good microbe vector
true
resident microorganisms in soil
gram positive, endospore forming
fungi
resident microorganisms in water
gram negaties
yeasts
moulds
resident microorganisms in animals and humans
abit of everything
if you have poor personal hygiene there’s usually more gram ______
negative
resident microorganisms in plants
yeasts and moulds
define sterile
free of living microorganisms
cannot be ‘quite sterile
define sterilisation
killing or removal of all viable microorganisms
3 killing mechanisms
heat
radiation
chemical
heat sterilisation must go above?
120 degrees
2 types of heat sterilisation
moist heat- steam aka autoclaving
dry heat- oven
dry heating is good for what?
oils
powders
most common form of chemical killing
ethylene oxide
what is the removal mechanism
filtration- with small pore sizes to retain the organism
TF: a whole product (incl packaging) can only have the same type of sterilisation
false, each component can have different ones
TF: sterilisation standards tell you how to do the sterilisation
no they just state what they expect
what must you validate to adhere to the sterilisation standards
the sterilising agent and the sterilising process
regulatory Bodies for sterilisation standards
european national standards
FDA
if the product is made in the UK but planned to be used in the US as well, which standards must it adhere to?
ENS
FDA
