Sterilisation 1

Question 1

2 methods of sterilisation

Answer 1

produce under clean conditions then terminally sterilise outer container- more common

produce and assemble under sterile conditions

Question 2

bacteria on synthetic materials?

Answer 2

low microbe content

get mostly from us handling them

Question 3

bacteria on semi-synthetic materials?

Answer 3

low microbe content, come mainly through us handling them

Question 4

bacteria on natural products?

Answer 4

have their own intrinsic microflora, vary depending on the products nature

Question 5

what is the primary requirement for bacterial growth

Answer 5

water

Question 6

TF: air is a good microbe vector

Answer 6

true

Question 7

resident microorganisms in soil

Answer 7

gram positive, endospore forming

fungi

Question 8

resident microorganisms in water

Answer 8

gram negaties
yeasts
moulds

Question 9

resident microorganisms in animals and humans

Answer 9

abit of everything

Question 10

if you have poor personal hygiene there’s usually more gram ______

Answer 10

negative

Question 11

resident microorganisms in plants

Answer 11

yeasts and moulds

Question 12

define sterile

Answer 12

free of living microorganisms

cannot be ‘quite sterile

Question 13

define sterilisation

Answer 13

killing or removal of all viable microorganisms

Question 14

3 killing mechanisms

Answer 14

heat
radiation
chemical

Question 15

heat sterilisation must go above?

Answer 15

120 degrees

Question 16

2 types of heat sterilisation

Answer 16

moist heat- steam aka autoclaving

dry heat- oven

Question 17

dry heating is good for what?

Answer 17

oils

powders

Question 18

most common form of chemical killing

Answer 18

ethylene oxide

Question 19

what is the removal mechanism

Answer 19

filtration- with small pore sizes to retain the organism

Question 20

TF: a whole product (incl packaging) can only have the same type of sterilisation

Answer 20

false, each component can have different ones

Question 21

TF: sterilisation standards tell you how to do the sterilisation

Answer 21

no they just state what they expect

Question 22

what must you validate to adhere to the sterilisation standards

Answer 22

the sterilising agent and the sterilising process

Question 23

regulatory Bodies for sterilisation standards

Answer 23

european national standards

FDA

Question 24

if the product is made in the UK but planned to be used in the US as well, which standards must it adhere to?

Answer 24

ENS

FDA