Steri Ppt Pt 2 Flashcards
Arnold’s sterilizer is used in
Tyndallization
Lowenstein Jensen is used in
TB Culture and inspissation
LF unglazed porcelian
Chamberland-Pasteur
Sterilization of high protein media
Inspissation; denaturing protein
75-80 C for 2hrs on three consec days
Inspissation
Separate bacteria from liquid or air
Filtration
Millipore fillers are also called
Cellulose esters
LF (liquid filtration) Seitz
Asbestos pad
___ used for critical sterilization
0.22 um
HEPA air filtration
High Efficiency Particulate Air filter
Most common biological safety cabinet
Class 2
Class 2A and Class 2B cabinet
Fixed opening, variable sash opening
Most common sterilant
Ethylene oxide
“Cold sterilization”
Ethylene oxide
Used for surgical appliances
2% glutaraldehyde
Effective in presence of organic matter
Peracetic acid
Eliminate food borne pathogens
Pasteurization
Tincture and iodophores fall under
Halogens
Dessication is also
Dehydration
Plasmolysis is
Lysis of cell membrane
Put agar in ref is
Bacteriostatic
2 aldehydes in chem. Methdo
2% glutaraldehyde, formaldehyde
In what form is chloride used in chem method
Hypochlorite
Effective against gram positive
Chloroxylenol
Alcohol and iodine solution for antiseptics
Tincture
Quats meaning
Quaternary Ammonium Compounds
MoAction of wuats
Protein denaturation and cell membrane destruction
Commonly used in surgery antiseptic
Chlorhexidine
Benzalkonium chloride inactivates
QUATS
MoA enzyme inhibition
Hexachlorophene
Povidone iodine is an
Iodophores
Vegetative - germinate spores - vegetative cells
Tyndallization