Steps in Tissue Preparation for Microscopic Study

Question 1

Fourth Step in Tissue Preparation for Microscopic Study is:

Answer 1

Dehydration

Question 2

The Seconed Step in Tissue Preparation for Microscopic Study is:

Answer 2

Cutting

Question 3

Seventh Step in Tissue Preparation for Microscopic Study is:

Answer 3

Sectioning

Question 4

The First Step in Tissue Preparation for Microscopic Study is:

Answer 4

Collection of Tissue

Question 5

The Fifth Step in Tissue Preparation for Microscopic Study is:

Answer 5

Clearing

Question 6

The ninth Step in Tissue Preparation for Microscopic Study is:

Answer 6

Mounting

Question 7

The third Step in Tissue Preparation for Microscopic Study is:

Answer 7

Fixation

Question 8

The sixth Step in Tissue Preparation for Microscopic Study is:

Answer 8

Embedding

Question 9

The eighth Step in Tissue Preparation for Microscopic Study is:

Answer 9

Staining

Question 10

The reason of collection of tissue soon after death:

Answer 10

To prevent the degradation of the tissue

Question 11

Why the thickness should not exceed .5cm (Light) & 1-2-mm (Electron)?

Answer 11

Because fixative may not penetrate whole tissue

Question 12

The cemicals that interact with amine groups or Cross-link with Proteins to fix the tissue as in living state as possible are:

Answer 12

Fixatives

Question 13

There are fixatives for Enzymatic Studies & During Surgical Procedures, true or false?

Answer 13

false

Question 14

The reason of we will not use fixative while we studying enzymatic activity is:

Answer 14

Because fixation will kill the specimen

Question 15

The reason of we will not use fixative during surgical procedures is:

Answer 15

Because we don’t have enough time to make this preparation and we cut the route by using liquid nitrogen

Question 16

Removal of water by immersing tissue block in gradual concentration of Alcohol means

Answer 16

Dehydration:

Question 17

Why we use dehydration in steps of Tissue Preparation for Microscopic Study?

Answer 17

To prevent it from mixing with the paraffin in the next step

Question 18

why immersing tissue block in gradual concentration of Alcohol is gradual?

Answer 18

Because we don’t need to harm the tissue by suddenly dehydration of it

Question 19

Removal of Alcohol with Xylene:

Answer 19

Clearing

Question 20

Why we use clearing in steps of Tissue Preparation for Microscopic Study?

Answer 20

To prevent Alcohol from mixing with the Paraffin & to give the specimen transparent look

Question 21

Maintain internal architect of the tissue and provide the block with certain solidity to facilitate its sectioning:

Answer 21

Embedding

Question 22

Sectioning by:

Answer 22

Use of MicrotomeUse of Microtome

Question 23

Basophilic means love acidic, true or false?

Answer 23

false

Question 24

Putting the specimen on Glass Slides and then covering it by chip of glass:

Answer 24

Mounting