Steps In Recombinant DNA Technology Flashcards
He discovered the Restriction enzymes in 1968
Werner Arber
A piece of DNA that has been created by the combination of at least two different DNA strands
Recombinant DNA (rDNA)
Joining together of DNA molecules from two different species
Recombinant DNA technology
Dna exist within the cell membrane along with other macromolecules such as Rna, polyssharides, proteins and lipids
Isolation of genetic material
the technique Agarose gel electrophoresis reveals the progress of the restriction enzyme digestion
Restrictions enzymes digestion
this technique involves running out the dna on an
Agarose gel
is a method of making multiple copies of a dna sequence using the enzyme
Amplification using PCR
Dna to be amplified
Template
small chemically synthesized oligonucleotides that are complementary to a region of the dna
Primer
DNA polymerase
Enzyme
Needed to extend the primers by the enzyme
Nucleotides
purified dna and the vector of interest are cut with the same restriction enzyme
Ligation of Dna molecules
joining these two pieces together using the enzyme dna ligase is
Ligation
intermixing of different dna strands is called
Recombination
the recombinant dna is introduced into a recipient host
Insertion of recombinant DNA into host
the recombinant dna is introduced into a recipient host this process is called
Transformation
3 types of insertion of recombinant DNA into host
Thermal shock
Calcium ion treatment
Electroporation
a marker gene of the plasmid vector is employed
Isolation of recombinant cells
the transformation process generates a mixed population of transform and non transformed host cells
Isolation of recombinant cell
the ultimate aim is to produce a desirable protein expression
Obtaining or cultural the foreign gene product
when you insert a piece of alien dna into a cloning vector and transfer it into a bacterial cells the alien dna gets multiplied
Obtaining or cultural the foreign gene product
genetically modified bacteria in the production of new types of experimental mutant mice for research purposes are someone to leading examples of genetic engineering in health
synthesis of synthetic human insulin and erythropoietin
