Steps In Recombinant DNA Technology

Question 1

He discovered the Restriction enzymes in 1968

Answer 1

Werner Arber

Question 2

A piece of DNA that has been created by the combination of at least two different DNA strands

Answer 2

Recombinant DNA (rDNA)

Question 3

Joining together of DNA molecules from two different species

Answer 3

Recombinant DNA technology

Question 4

Dna exist within the cell membrane along with other macromolecules such as Rna, polyssharides, proteins and lipids

Answer 4

Isolation of genetic material

Question 5

the technique Agarose gel electrophoresis reveals the progress of the restriction enzyme digestion

Answer 5

Restrictions enzymes digestion

Question 6

this technique involves running out the dna on an

Answer 6

Agarose gel

Question 7

is a method of making multiple copies of a dna sequence using the enzyme

Answer 7

Amplification using PCR

Question 8

Dna to be amplified

Answer 8

Template

Question 9

small chemically synthesized oligonucleotides that are complementary to a region of the dna

Answer 9

Primer

Question 10

DNA polymerase

Answer 10

Enzyme

Question 11

Needed to extend the primers by the enzyme

Answer 11

Nucleotides

Question 12

purified dna and the vector of interest are cut with the same restriction enzyme

Answer 12

Ligation of Dna molecules

Question 13

joining these two pieces together using the enzyme dna ligase is

Answer 13

Ligation

Question 14

intermixing of different dna strands is called

Answer 14

Recombination

Question 15

the recombinant dna is introduced into a recipient host

Answer 15

Insertion of recombinant DNA into host

Question 16

the recombinant dna is introduced into a recipient host this process is called

Answer 16

Transformation

Question 17

3 types of insertion of recombinant DNA into host

Answer 17

Thermal shock
Calcium ion treatment
Electroporation

Question 18

a marker gene of the plasmid vector is employed

Answer 18

Isolation of recombinant cells

Question 19

the transformation process generates a mixed population of transform and non transformed host cells

Answer 19

Isolation of recombinant cell

Question 20

the ultimate aim is to produce a desirable protein expression

Answer 20

Obtaining or cultural the foreign gene product

Question 21

when you insert a piece of alien dna into a cloning vector and transfer it into a bacterial cells the alien dna gets multiplied

Answer 21

Obtaining or cultural the foreign gene product

Question 22

genetically modified bacteria in the production of new types of experimental mutant mice for research purposes are someone to leading examples of genetic engineering in health

Answer 22

synthesis of synthetic human insulin and erythropoietin