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HISTOPATHOLOGY > Standard Manual Processing Technique > Flashcards

Standard Manual Processing Technique Flashcards

1
Q

FDD-CIET-SSML

A

Fixation preservation
Decalcification optional
Dehydration dessication
Clearning dealcoholization
Impregnation infiltration
Embedding casting/blocking
Trimming
Ssectioning section cutting
Staining
Mounting
Labelling

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2
Q

Fixing agent for 24 HOURS
Tissue to volume ratio 1:20

A

FIXATION

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3
Q

Arrange the secondary signs of death in order:

  1. Livor mortis
  2. Autolysis
  3. Algor mortis
  4. Rigor mortis
  5. Putrefaction
  6. Dessication
  7. Postmortem clotting
A

3, 4, 1, 2, 5, 6, 7

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4
Q

Teasing is the process wherein selected specimen is immersed in a watch glass containing _______________________, carefully dissected and examined under a microscope, either unstained by Phase contrast microscope or satined by bright field microscope.

A

ISOTONIC SALT SOLUTION
NSS
RINGER’S LACTATE SOLUTION

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5
Q

Other term for teasing

A

dissociation

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6
Q

Tissue exam normally utilized when a RAPID DIAGNOSIS of the tissue in question is required

A

Frozen section

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7
Q

Frozen section is recommended for ________ and _______ tissue demonstration

A

LIPIDS, NERVOUS

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8
Q

Other term for squash preparation

A

CRUSHING

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9
Q

chicken fatformation and Currant jelly fat like formation

A

Postmortem clotting

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10
Q

Primary goal of Fixation

A

To PRESERVE the morphologic and chemical integrity of the cell

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11
Q

Secondary purpose of Fixation

A

To Harden and Protect tissue

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12
Q

Most important reaction in fixation

A

Protein stabilization

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13
Q

This causes cell to swell

A

Tissue in water hypotonic solution

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14
Q

This causes cell to shrink

A

Tissue in strong salt (hypertonic solution

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15
Q

Formalin penetrates tissue at a rate of

A

1mm/hr

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16
Q

Fixation is NOT Required/Recommended when tissues will be used for microbial and toxicogical studies because

A

Fixation disables culture growth (kills bacteria)

Fixation neutralizes drugs and toxins

17
Q

Ideal number of hours for fixation

A

6 to 18 hours

HISTOPATHOLOGY (8 decks)

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