Stains And Culture Media

Question 1

Groccot hexamine silver stains? And what colour?

Answer 1

Stains fungi and acanthamoeba (black)

Question 2

Alcian blue stains?

Answer 2

Mucopolysaccharide (as seen in macular dystrophy of the cornea)

Question 3

Oil red O stains?

Answer 3

Lipid red (sebaceous cell carcinoma, Schnyder dystrophy)

Question 4

Prussian blue stains?

Answer 4

Iron (which is usually intracellular)

Question 5

Ziehl Neilson stains?

Answer 5

Mycobacteria

Question 6

Alizarin red stains?

Answer 6

Calcium red

Question 7

Von Kossa stains?

Answer 7

Calcium black

Question 8

Masson Trichrome stains?

Answer 8

New collagen blue (Reis-Buckler)
And hyaline material red (granular dystrophy)

Question 9

Congo red stains?

Answer 9

Amyloid red

Question 10

Haematoxylin stains?

Answer 10

Acid substances PURPLE

Question 11

Eosin stains?

Answer 11

Basic substances pink

Question 12

Thayer Martin medium…

Answer 12

Grows gram negative cocci (Neisseria)

Question 13

MacConkey agar…

Answer 13

…grows lactose fermenting gram negatives (enteric bacteria)

Question 14

Blood agar…

Answer 14

…grows most bacteria (except Haemophilus, Neisseria, Moraxella) and fungi

Question 15

Cooked meat broth…

Answer 15

…grows anaerobes and fastidious organisms

Question 16

Thioglycate agar…

Answer 16

…grows aerobes on the surface and anaerobes inside

Question 17

Lowenstein-Jensen…

Answer 17

… grows Mycobacteria tuberculosis

Question 18

McCoy agar…

Answer 18

…grows obligate intracellular bacteria (ex.: Chlamydia)

Question 19

Non-nutrient agar with E. coli…

Answer 19

…grows Acanthamoeba

Question 20

Sabouraud agar…

Answer 20

…grows fungi

Question 21

Brain heart infusion…

Answer 21

Grows streptococci and meningococci

Question 22

Non nutrient agar with E coli overaly

Answer 22

Acanthamoeba spp.

Question 23

Chocolate agar…

Answer 23

Grows Moraxella, Neisseria and Haemophilus