stains Flashcards
all the stains in histology workbook
What does Alcian blue stain blue?
GAG-rich structures, mucous goblet cells, mast cell granules and cartilage matrix
What does Eosin stain pink?
Colloidal proteins
What does Eosin stain orange/red?
Keratin
What does Iron Haematoxylin stain black?
Nuclei/Elastic fibres
What does Periodic Acid Schiff (PAS) stain magenta?
Hexose sugars (especially complex carbs- goblet cell mucin, cartilage matrix, glycogen, basement membrane, glycocalyx)
What does Perl’s stain stain Prussian blue?
Ferric iron
What does Romanovsky stain (Giemsa’s of Leishman’s) stain purple?
Nuclei, Azurophils, Neutrophil granules
What does Romanovsky stain (Giemsa’s of Leishman’s) stain red/pink?
Erythrocytes & eosinophil granules
What does Romanovsky stain (Giemsa’s of Leishman’s) stain blue?
Lymphocyte/monocyte cytoplasm, basophil granules
What does Toluidine blue stain dark blue?
Nuclei, Ribosomes, Cytoplasm
What does Toluidine blue stain pale blue?
Cartilage matrix, mast cell granules
What does Toluidine blue stain purple?
GAG rich components
What does Van Gieson’s trichrome (with haemolytic counterstain) stain pink/red?
Collagen
What does Van Gieson’s trichrome (with haemolytic counterstain) stain yellow/olive green?
Cell cytoplasm
What does Van Gieson’s trichrome (with haemolytic counterstain) stain black?
Nuclei
What colour does Alcian blue stain GAG rich structures, mucous goblet cells, mast cell granules and the cartilage matrix?
Blue
What colour does eosin stain colloidal proteins?
Pink
What colour does eosin stain keratin?
Orange/red
What colour does iron haematoxylin stain nuclei and elastic fibres?
Black
What colour does haematoxylin stain nuclei/RNA?
Blue
What does haematoxylin stain blue?
Nuclei/RNA
What colour does periodic acid schiff (PAS) stain hexose sugars?
Magenta
What colour does Perls stain turn ferric iron
Prussian blue
What colour does Romanovsky stain turn chromatin, azurophils & neutrophil granules?
Purple
What colour does Romanovsky stain turn erythrocytes & eosinophil granules?
Red/pink
What colour does Romanovsky stain turn lymphocyte/monocyte cytoplasm and basophil granules?
Dark blue/purple
What colour does Toluidine blue stain nuclei, ribosomes, and cytoplasm?
Dark blue
What colour does Toluidine blue stain the cartilage matrix, mast cell granules and GAG rich compounds?
Bright purple
What colour does Van Gieson’s trichrome stain turn collagen?
Pink/red
What colour does Van Gieson’s trichrome stain turn cell cytoplasm?
Yellow/olive green
What colour does Van Gieson’s trichrome stain turn nuclei?
Black
What is H&E used to see, and what colours do they stain?
Cytoplasm-pink, nuclei-blue
