stains Flashcards

all the stains in histology workbook

1
Q

What does Alcian blue stain blue?

A

GAG-rich structures, mucous goblet cells, mast cell granules and cartilage matrix

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2
Q

What does Eosin stain pink?

A

Colloidal proteins

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3
Q

What does Eosin stain orange/red?

A

Keratin

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4
Q

What does Iron Haematoxylin stain black?

A

Nuclei/Elastic fibres

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5
Q

What does Periodic Acid Schiff (PAS) stain magenta?

A

Hexose sugars (especially complex carbs- goblet cell mucin, cartilage matrix, glycogen, basement membrane, glycocalyx)

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6
Q

What does Perl’s stain stain Prussian blue?

A

Ferric iron

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7
Q

What does Romanovsky stain (Giemsa’s of Leishman’s) stain purple?

A

Nuclei, Azurophils, Neutrophil granules

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8
Q

What does Romanovsky stain (Giemsa’s of Leishman’s) stain red/pink?

A

Erythrocytes & eosinophil granules

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9
Q

What does Romanovsky stain (Giemsa’s of Leishman’s) stain blue?

A

Lymphocyte/monocyte cytoplasm, basophil granules

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10
Q

What does Toluidine blue stain dark blue?

A

Nuclei, Ribosomes, Cytoplasm

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11
Q

What does Toluidine blue stain pale blue?

A

Cartilage matrix, mast cell granules

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12
Q

What does Toluidine blue stain purple?

A

GAG rich components

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13
Q

What does Van Gieson’s trichrome (with haemolytic counterstain) stain pink/red?

A

Collagen

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14
Q

What does Van Gieson’s trichrome (with haemolytic counterstain) stain yellow/olive green?

A

Cell cytoplasm

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15
Q

What does Van Gieson’s trichrome (with haemolytic counterstain) stain black?

A

Nuclei

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16
Q

What colour does Alcian blue stain GAG rich structures, mucous goblet cells, mast cell granules and the cartilage matrix?

17
Q

What colour does eosin stain colloidal proteins?

18
Q

What colour does eosin stain keratin?

A

Orange/red

19
Q

What colour does iron haematoxylin stain nuclei and elastic fibres?

20
Q

What colour does haematoxylin stain nuclei/RNA?

21
Q

What does haematoxylin stain blue?

A

Nuclei/RNA

22
Q

What colour does periodic acid schiff (PAS) stain hexose sugars?

23
Q

What colour does Perls stain turn ferric iron

A

Prussian blue

24
Q

What colour does Romanovsky stain turn chromatin, azurophils & neutrophil granules?

25
What colour does Romanovsky stain turn erythrocytes & eosinophil granules?
Red/pink
26
What colour does Romanovsky stain turn lymphocyte/monocyte cytoplasm and basophil granules?
Dark blue/purple
27
What colour does Toluidine blue stain nuclei, ribosomes, and cytoplasm?
Dark blue
28
What colour does Toluidine blue stain the cartilage matrix, mast cell granules and GAG rich compounds?
Bright purple
29
What colour does Van Gieson's trichrome stain turn collagen?
Pink/red
30
What colour does Van Gieson's trichrome stain turn cell cytoplasm?
Yellow/olive green
31
What colour does Van Gieson's trichrome stain turn nuclei?
Black
32
What is H&E used to see, and what colours do they stain?
Cytoplasm-pink, nuclei-blue