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histology > stains > Flashcards

stains Flashcards

all the stains in histology workbook

1
Q

What does Alcian blue stain blue?

A

GAG-rich structures, mucous goblet cells, mast cell granules and cartilage matrix

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2
Q

What does Eosin stain pink?

A

Colloidal proteins

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3
Q

What does Eosin stain orange/red?

A

Keratin

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4
Q

What does Iron Haematoxylin stain black?

A

Nuclei/Elastic fibres

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5
Q

What does Periodic Acid Schiff (PAS) stain magenta?

A

Hexose sugars (especially complex carbs- goblet cell mucin, cartilage matrix, glycogen, basement membrane, glycocalyx)

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6
Q

What does Perl’s stain stain Prussian blue?

A

Ferric iron

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7
Q

What does Romanovsky stain (Giemsa’s of Leishman’s) stain purple?

A

Nuclei, Azurophils, Neutrophil granules

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8
Q

What does Romanovsky stain (Giemsa’s of Leishman’s) stain red/pink?

A

Erythrocytes & eosinophil granules

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9
Q

What does Romanovsky stain (Giemsa’s of Leishman’s) stain blue?

A

Lymphocyte/monocyte cytoplasm, basophil granules

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10
Q

What does Toluidine blue stain dark blue?

A

Nuclei, Ribosomes, Cytoplasm

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11
Q

What does Toluidine blue stain pale blue?

A

Cartilage matrix, mast cell granules

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12
Q

What does Toluidine blue stain purple?

A

GAG rich components

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13
Q

What does Van Gieson’s trichrome (with haemolytic counterstain) stain pink/red?

A

Collagen

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14
Q

What does Van Gieson’s trichrome (with haemolytic counterstain) stain yellow/olive green?

A

Cell cytoplasm

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15
Q

What does Van Gieson’s trichrome (with haemolytic counterstain) stain black?

A

Nuclei

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16
Q

What colour does Alcian blue stain GAG rich structures, mucous goblet cells, mast cell granules and the cartilage matrix?

A

Blue

17
Q

What colour does eosin stain colloidal proteins?

A

Pink

18
Q

What colour does eosin stain keratin?

A

Orange/red

19
Q

What colour does iron haematoxylin stain nuclei and elastic fibres?

A

Black

20
Q

What colour does haematoxylin stain nuclei/RNA?

A

Blue

21
Q

What does haematoxylin stain blue?

A

Nuclei/RNA

22
Q

What colour does periodic acid schiff (PAS) stain hexose sugars?

A

Magenta

23
Q

What colour does Perls stain turn ferric iron

A

Prussian blue

24
Q

What colour does Romanovsky stain turn chromatin, azurophils & neutrophil granules?

A

Purple

25
Q

What colour does Romanovsky stain turn erythrocytes & eosinophil granules?

A

Red/pink

26
Q

What colour does Romanovsky stain turn lymphocyte/monocyte cytoplasm and basophil granules?

A

Dark blue/purple

27
Q

What colour does Toluidine blue stain nuclei, ribosomes, and cytoplasm?

A

Dark blue

28
Q

What colour does Toluidine blue stain the cartilage matrix, mast cell granules and GAG rich compounds?

A

Bright purple

29
Q

What colour does Van Gieson’s trichrome stain turn collagen?

A

Pink/red

30
Q

What colour does Van Gieson’s trichrome stain turn cell cytoplasm?

A

Yellow/olive green

31
Q

What colour does Van Gieson’s trichrome stain turn nuclei?

A

Black

32
Q

What is H&E used to see, and what colours do they stain?

A

Cytoplasm-pink, nuclei-blue

histology (3 decks)

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