STAINS Flashcards

1
Q

Hematoxylin

A

Negatively charged

Nucleus

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2
Q

Eosin

A

Positively charged

Cytoplasm

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3
Q

Oil Red O stain

A

Fat/ Lipid

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4
Q

Sudan black B
Sudan IV
Annexin V
Osmium Tetroxide

A

Fat and Lipid

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5
Q

PAS stain

A
Glycogen
Fungus and amoeba
Basement membrane
Lymphoblast
Erythroblast
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6
Q

Von Kossa stain

m/c used

A

Calcium

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7
Q

Alizarin stain

m/specific

A

Calcium in tissue

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8
Q

Calcein

A

Calcium

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9
Q

Perl’s Prussiun blue

A

Iron/ Hemosiderin

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10
Q

Gomori’s metbemine silver stain

A

Best Fungal stain

stains both live and dead fungus

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11
Q

Lugols Iodine

A

Amyloid stain (Gross specimen)

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12
Q

Congo RED stain

A

Pink / red (in Ordinary Light )

Apple Green birefringence (in Polarised light)

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13
Q

Alcian Blue

A

Mucopolysaccharide acid

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14
Q

Mayer’s mucaraminestain

A

Sialomucin

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15
Q

Acid Fast stain

A

Mycobacterium

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16
Q

Fontana Masson stain

A

Melanin and Argentaffin cells

17
Q

Verhoff Von Gelson (VVG)

A

Elastic fibres

18
Q

Orcein stain

A

Elastic fibres
Hepatitis B surface antigens
Copper assosciated proteins

19
Q

Shikata’s stain

A

Copper protein complexes

20
Q

Rhodamine stain (best)

A

Copper in tissue

21
Q

Toludine Blue

A

Mast cells

22
Q

Luxol Fast Blue

A

Myelin and Nissil substances

23
Q

Bieschowsky stain

A

Neuronal axons
Neurofibriary tangles
Neuritic plaques

24
Q

Picro Mallory stain

Lendrums method

A

Fibrin

25
Q

Phospho Tungstic Acid Hematoxylin (PTAH) stain

A

Muscle Fibres

collagen

26
Q

Russel Movat Pentachrome stain

A

Muscle
Elastic fibres
Collagen/ Reticular fibres

27
Q

Ethyl green Pyronine stain

A

Differential demonstration of

DNA and RNA

28
Q

Fuelgen stain

A

DNA

29
Q

Hall’s Stain

A

Bilirubin

30
Q

Warthin Starry stain

A

Helicobacter pylori

31
Q

Leder stain

A

Marker of Neutrophil