Stains

Question 1

Masson-trichrome stain looks for…?

Answer 1

Mature collagen - blue; immature collagen, keratin, muscle, and nerves - red

Question 2

Van Gieson’s stain looks for…?

Answer 2

Mature collagen - red; immature collagen, keratin, muscle, and nerves - yellow

Question 3

Verhoff’s stain looks for…?

Answer 3

Elastin and nuclei - black

Question 4

Gimori’s aldehyde fuchsin looks for…?

Answer 4

Elastin, suflated acid mucopolysaccharides, and certain epithelial mucins - purple

Question 5

Oil red O stain looks for…?

Answer 5

Lipids - dark red

Question 6

Sudan black B stain looks for …?

Answer 6

Lipids - green/black

Question 7

Scarlet red stains for …?

Answer 7

Lipids - red

Question 8

Gomori’s or Wilder’s reticulin stain looks for…?

Answer 8

Reticulin, melanin, and nerves - dark brown to black

Question 9

Periodic acid schiff stain looks for …?

Answer 9

Glycogen, neutral mucopolysaccharides, fungi, and tissue debris - red

Question 10

Alcian blue stain looks for…?

Answer 10

Acid mucopolysaccharides - blue

Question 11

Hale’s colloidal iron stain looks for…?

Answer 11

Acid mucopolysaccharides - blue

Question 12

Toluidine blue stain looks for?

Answer 12

Acid mucopolysaccharides and mast cell granules - purple

Question 13

Gomori’s methenamine silver stain looks for?

Answer 13

Fungi and melanin - black

Question 14

Gram’s or Brown-Brenn stain looks for?

Answer 14

Gram-positive bacteria -blue; gram-negative bacteria - red

Question 15

Fite’s modified acid-fast stain looks for?

Answer 15

Acid-fast bacteria - red

Question 16

Fontana’s ammoniacal silver nitrate stain looks for?

Answer 16

Premelanin and melanin - black (hemosiderin usually positive too, but less intense)