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Levers > Stains > Flashcards

Stains Flashcards

1
Q

routine

A

H&E
nuclei: blue
collagen muscles and nerves: red

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2
Q

Collagen

A

Masson-Trichome
collagen: blue or green
nuclei, muscles, nerves: red

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3
Q

elastic fibers

A

Verhoeff-van Guesson
elastic: black
collagen: red
nuclei, muscles, nerves: yellow

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4
Q

elastic fibers

A

Pinkus acid orcein

elastic: dark brown

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5
Q

Melanin, reticulate fibers( argyrophillic)

A

silver nitrate

black

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6
Q

melanin (argentaffin)

A

Fontana Mason

black

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7
Q

fungi, Donovan bodies, Frisch bacilii (rhinoscleroma), basement membrane

A

Gomori methamenenamine silver GMS

black

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8
Q

fungi

A

Grocott

fungi cell wall: black

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9
Q

glycogen
neutral MPS
fungi

A

PAS
glycogen:red, diastate labile
neutral MPS, fungi: red, diastase resistant

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10
Q

acid MPS

A

alcian blue pH2.5

blue

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11
Q

sulfated MPS

A

alcian blue pH 0.5

blue

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12
Q

acid MPS

A

toluidine blue
colloidal iron

blue

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13
Q

hyaluronic acid

A

hyaluronidase

hyaluronidase labile

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14
Q

epithelial mucin

A

mucicarmine

red

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15
Q

mast cells granules, acid MPS, myeloid granules, Leishmania

A

Giemsa
MCG, acid MPS: metachromatically purple
myeloid granules, leishmania: red

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16
Q

acid fast bacilli

17
Q

hemosiderin

A

perl’s potassium ferrocyanide

blue

18
Q

amyloid

A

alkaline congo red

pink red, green birefringence on polarized light

19
Q

calcium

A

von kossa

black

20
Q

lipids

A

scarlet red
oil red o
red

21
Q

tyrosinase in melanocytes

A

Dopa

black dopa- melanin

22
Q

mast cells, neutrophils, myelocytes

A

naphthol-AS-D-chloroacetate esterase

granules are red

23
Q

spirochetes

A

warthin starry

black

24
Q

spirochetes, bacillary angiomatosis

A

dieterle and Steiner

black

25
Q

AFS

A

ziehl neilson

red

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