Stains Flashcards
Simply means coloring the microorganisms with a dye that emphasizes certain structures.
Staining
When a specimen is to be fixed, a thin film of material containing the microorganism is spread over the surface of the
slide. This film is called?
Smear
Stains are salts composed of a positive and negative ion, one of which is colored and is
known as the?
Chromophore
The color of so called basic dyes is in the?
positive ions
Colors of so called acidic dyes is in the?
Negative ion
Is the preparation of colorless bacteria against a colored background.
Negative staining
It is an aqueous or alcohol solution of a single basic dye. The primary purpose of a ___________is to
highlight the entire microorganism.
Simple stain
It is a chemical that is added to the solution to intensify the stain. The function of a________ is to
increase the affinity of a stain for a biological specimen.
Mordant
It reacts differently with different kinds of bacteria and can be used to distinguish among
them. The most frequently used for bacteria are the gram stain and acid-fast stain?
Differential stain
It was developed in 1884 by the Danish bacteriologist Hans Christian Gram.
Gram stain
Used to distinguish Mycobacterium species and some species of Nocardia.
Acid fast staining
It is used to color and isolate specific parts of microorganisms, such as endospores and flagella, and to reveal the
presence of capsules.
Special stains
It is used to demonstrate the presence of capsules. Because capsules do not accept most stains, the capsules
appear as unstained halos around bacterial cells and stand out against a dark background.
Negative staining for capsules.
The most commonly used endospore stain is the Schaeffer-fulton endospore stain.
Endoscope stainning
It is used to demonstrate the presence of flagella.
Flagella staining
