Stains

Question 1

Usual stains

Answer 1

Hx (blue)(basophilic structures)
E (pink)(acidophilus structures)

Question 2

CHO

Answer 2

Best’s carmine (red)
PAS (purple)

Question 3

Fat

Answer 3

Sudan III (orange)
Sudan black (black)
Osmic acid (black)

Question 4

Mitochondria

Answer 4

Acid fuscin (black)
Janus green (green)
Iron hematoxylin (black)

Question 5

Golgi body

Answer 5

Hx and E (negative golgi image)
Silver Ag (fibrils)

Question 6

Cell membrane

Answer 6

Silver Ag (dark brown)
PAS (purple)

Question 7

Cell coat

Answer 7

PAS (purple)
Best’s carmine (red)

Question 8

Lysosomes

Answer 8

Histochemical test (acid phosphatase)

Question 9

Microtubules

Answer 9

Immune fluorescent technique

Question 10

Centriole

Answer 10

Iron hematoxylen (small dark bodies)

Question 11

Cilia

Answer 11

Eosin (pink)(acidophilic)

Question 12

RER

Answer 12

Hx (blue)(basophilic)

Question 13

SER

Answer 13

Eosin (pink)(acidophilic)

Question 14

Nucleus/nucleolus/ribosomes/RER

Answer 14

All Basophilic so Hx (blue)

Question 15

Goblet cells

Answer 15

PAS
Best’s carmine

Question 16

Elastic fibers

Answer 16

Orcein
Van Gasion

Question 17

Reticular fibers

Answer 17

Silver
PAS/best’s

Question 18

Collagen fibres

Answer 18

Eosin (pink)

Question 19

Adipocytes

Answer 19

Sudan III (orange)
Sudan black (black)
Hx and E (signet ring structure)

Question 20

Mast cell granules

Answer 20

Toluidine blue (red or purple)(metachromatic)

Question 21

Barr body

Answer 21

Cresyl violet (in buccal mucosa)(dark mass in inner side of nuclear membrane)
Leishman (in blood leukocyte)(drumstick attached to their nuclei)