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Histopath > Staining > Flashcards

Staining Flashcards

1
Q

Uses aquaous or alcoholic dye solutions to produce color

A

Direct staining

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2
Q

Uses a mordant or another agent to intensify the action of dye used

A

Indirect staining

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3
Q

Serves as a link or bridge between the tissue and dye

A

Mordant

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4
Q

Hastens the staining rxn by increasing the staining power and selectivity of the dye

A

Accentuator

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5
Q

No overstaining. No decolorizer. Specific periods of time or until desired color is attained

A

Progressive staining

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6
Q

Overstaing.decolorized until desired color is attained

A

Regressive

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7
Q

Selective romoval of excess stain so that a specific subs. May stain distinctly from the surrounding tissue

A

Decolorizer/Differentiation

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8
Q

Staining with a color that is different from that of the stain itself. Water is important in this procedure

A

Metachromatic staining

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9
Q

For contrast and background. Stain wid a diff color taht if the primary stain

A

Counterstaining

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10
Q

Demonstration of tissue elements using solutions of metallic salts taht are deposited on the tissue surface

A

Metallic impregnation

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11
Q

Selective staining of living cell constituents . Demo cutoplasmic structures

A

Vital stains

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12
Q

It is not itself a stainx hematin is the active coloring. Ontained by ripening—ocidation of hematoxylin

A

Hematoxylin

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13
Q

Extarcted from cocchus cacti (female cochneal bug)

A

Cochineal dye

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14
Q

Carmine dye . Chromatin and nuclear stain for fresh and smear prep

A

Cochineal dye with alum

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15
Q

For neuropathological stain

A

Coch. With picric acid–picrocarmine

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16
Q

Demonstration of glycogen. With aluminum chloride

A

Best’s carmine

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17
Q

Stains the cytoplasm and nucleus simultaneously and differentially. Insoluble to barely soluble in water

A

Neutral dye

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18
Q

Example of a neutral dye

A

Romanowsky dyes

19
Q

Extracted from lichens. Staining for elastic fibers . Source of litmus paper

A

Orcein

20
Q

Coal tar dyes or aniline dyes

A

Synthetic dyes

21
Q

Routinely used in H and E stain. It initially stains the nuclei reddish

A

Aluminum hematoxylin

22
Q

Nuclear staining in paps

A

Harris hematoxylin

23
Q

Excellent nuclear stain . Stain mucins recommended for bone and cartilage

A

Erlich’s hematoxylin

24
Q

Chemically ripend by sodium iodine.

A

Mayer’s hematoxylin

25
Q

Uses iron as both as a mordant and a ripening agent

A

Iron hematoxylin

26
Q

Gray-black. Nuclear and cytoplasmic inclusions. Iron alum as a modant/oxidizer

A

Heindenhain’s hematoxylin

27
Q

Standard iron hematoxylin. Ferric ammonium chloride is mordant/oxidizer

A

Weigert’s hematoxylin

28
Q

Natural ripening achieved eith light and air. For cns , general tissue

A

Phosphotungstic acid hematoxylin

29
Q

A red acid dye. Routinely used as a counterstain. Stains connective tissue and cytoplasm differentially

A

Eosin

30
Q

Most common eosin. Soluble in water. Green yellow fl

A

Eosin Y

31
Q

Deeper red color eosin

A

Eosin B

32
Q

Eosin soluble in alcohol

A

Eosin S

33
Q

For staining hgb

A

Benzidine

34
Q

Staining diptheria org. And used as a counterstain in grams acid fast and paps

A

Bismarck brown

35
Q

Discriminates dead and living cells . Dna -green Rna-red

A

Acridine orange (masson stain)

36
Q

Used for rountine staining of fixed sections. Resistant to strong acid dyes

A

Celestine blue

37
Q

Best known as a indicator. Stains elastic tissues, amyloid, myelin

A

Congo red

38
Q

A nuclear or chromatin stain. Stains amyloid in rfs, and plts

A

Crystal violet

39
Q

Stain blood to differentiate wbc’s–for blood parasites

A

Giemsa stain

40
Q

Oldest stain.

A

Iodine

41
Q

Stain mitochondria during supravital

A

Janus green B

42
Q

Counterstain for ascaris eggs rbcs bacterial spores –fulton scheffer
Counterstain for acid fast

A

Malachite green

43
Q

Best vital dye . Basic dye

A

Neutral red

44
Q

Used to stain fats (black) permanent

A

Osmium tetroxide

Histopath (1 decks)

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