Staining

Question 1

Uses aquaous or alcoholic dye solutions to produce color

Answer 1

Direct staining

Question 2

Uses a mordant or another agent to intensify the action of dye used

Answer 2

Indirect staining

Question 3

Serves as a link or bridge between the tissue and dye

Answer 3

Mordant

Question 4

Hastens the staining rxn by increasing the staining power and selectivity of the dye

Answer 4

Accentuator

Question 5

No overstaining. No decolorizer. Specific periods of time or until desired color is attained

Answer 5

Progressive staining

Question 6

Overstaing.decolorized until desired color is attained

Answer 6

Regressive

Question 7

Selective romoval of excess stain so that a specific subs. May stain distinctly from the surrounding tissue

Answer 7

Decolorizer/Differentiation

Question 8

Staining with a color that is different from that of the stain itself. Water is important in this procedure

Answer 8

Metachromatic staining

Question 9

For contrast and background. Stain wid a diff color taht if the primary stain

Answer 9

Counterstaining

Question 10

Demonstration of tissue elements using solutions of metallic salts taht are deposited on the tissue surface

Answer 10

Metallic impregnation

Question 11

Selective staining of living cell constituents . Demo cutoplasmic structures

Answer 11

Vital stains

Question 12

It is not itself a stainx hematin is the active coloring. Ontained by ripening—ocidation of hematoxylin

Answer 12

Hematoxylin

Question 13

Extarcted from cocchus cacti (female cochneal bug)

Answer 13

Cochineal dye

Question 14

Carmine dye . Chromatin and nuclear stain for fresh and smear prep

Answer 14

Cochineal dye with alum

Question 15

For neuropathological stain

Answer 15

Coch. With picric acid–picrocarmine

Question 16

Demonstration of glycogen. With aluminum chloride

Answer 16

Best’s carmine

Question 17

Stains the cytoplasm and nucleus simultaneously and differentially. Insoluble to barely soluble in water

Answer 17

Neutral dye

Question 18

Example of a neutral dye

Answer 18

Romanowsky dyes

Question 19

Extracted from lichens. Staining for elastic fibers . Source of litmus paper

Answer 19

Orcein

Question 20

Coal tar dyes or aniline dyes

Answer 20

Synthetic dyes

Question 21

Routinely used in H and E stain. It initially stains the nuclei reddish

Answer 21

Aluminum hematoxylin

Question 22

Nuclear staining in paps

Answer 22

Harris hematoxylin

Question 23

Excellent nuclear stain . Stain mucins recommended for bone and cartilage

Answer 23

Erlich’s hematoxylin

Question 24

Chemically ripend by sodium iodine.

Answer 24

Mayer’s hematoxylin

Question 25

Uses iron as both as a mordant and a ripening agent

Answer 25

Iron hematoxylin

Question 26

Gray-black. Nuclear and cytoplasmic inclusions. Iron alum as a modant/oxidizer

Answer 26

Heindenhain's hematoxylin

Question 27

Standard iron hematoxylin. Ferric ammonium chloride is mordant/oxidizer

Answer 27

Weigert's hematoxylin

Question 28

Natural ripening achieved eith light and air. For cns , general tissue

Answer 28

Phosphotungstic acid hematoxylin

Question 29

A red acid dye. Routinely used as a counterstain. Stains connective tissue and cytoplasm differentially

Answer 29

Eosin

Question 30

Most common eosin. Soluble in water. Green yellow fl

Answer 30

Eosin Y

Question 31

Deeper red color eosin

Answer 31

Eosin B

Question 32

Eosin soluble in alcohol

Answer 32

Eosin S

Question 33

For staining hgb

Answer 33

Benzidine

Question 34

Staining diptheria org. And used as a counterstain in grams acid fast and paps

Answer 34

Bismarck brown

Question 35

Discriminates dead and living cells . Dna -green Rna-red

Answer 35

Acridine orange (masson stain)

Question 36

Used for rountine staining of fixed sections. Resistant to strong acid dyes

Answer 36

Celestine blue

Question 37

Best known as a indicator. Stains elastic tissues, amyloid, myelin

Answer 37

Congo red

Question 38

A nuclear or chromatin stain. Stains amyloid in rfs, and plts

Answer 38

Crystal violet

Question 39

Stain blood to differentiate wbc's--for blood parasites

Answer 39

Giemsa stain

Question 40

Oldest stain.

Answer 40

Iodine

Question 41

Stain mitochondria during supravital

Answer 41

Janus green B

Question 42

Counterstain for ascaris eggs rbcs bacterial spores --fulton scheffer Counterstain for acid fast

Answer 42

Malachite green

Question 43

Best vital dye . Basic dye

Answer 43

Neutral red

Question 44

Used to stain fats (black) permanent

Answer 44

Osmium tetroxide