Staining

Question 1

What fixative must not be used before H&E staining method?

Answer 1

Osmic acid solutions

Question 2

For tissues fixed with mercurice chloride, staining time of hematoxylin should be ____________ slightly while duration of eosin staining should be ____________.

Answer 2

increased; reduced

Question 3

What is used to remove mercury?

Answer 3

Iodine-alcohol

Question 4

What is used to remove iodine?

Answer 4

sodium thiosulfate and wash with water

Question 5

The process of attaching sections by coating the side with dilute or thin celloidin.

Answer 5

Colloidionization

Question 6

Production of formation of colors of different tissues and cells.

Answer 6

staining

Question 7

Who attempted the use of saffron for staining?

Answer 7

Anton van Leeuwenhoek

Question 8

Used carmine staining to diffuse colouration of tissues.

Answer 8

Goppert and Cohn

Question 9

Who attempted the first use of selective nuclear stain.

Answer 9

Gerlach

Question 10

What staining reaction occurs when tissue is penetrated by a dye solution?

Answer 10

absorption

Question 11

What staining reaction is occurring when there’s an accumulation in the surface of the compound?

Answer 11

Adsorption

Question 12

Sections are stained with a simple aqueous or alcoholic solution of the dye.

Answer 12

Direct staining

Question 13

Requires the use of a mordant or accentuator in order to produce the desired density or intensity of color.

Answer 13

Indirect staining

Question 14

A substance which serves as a link or bridge to form a colored lake.

Answer 14

Mordant

Question 15

Accelerates or happens the speed of the staining reaction by increasing the staining power and selectivity of the dye.

Answer 15

Accentuator

Question 16

Once the tissue is stained, it is not washed or decolorized. May result in diffused and obscuring effect.

Answer 16

Progressive staining

Question 17

The tissue is first overstained to obliterate the tissue and excess stain is removed or decolorized.

Answer 17

Regenerative staining

Question 18

Uses specific dyes which differentiate particular substances by staining them with a color different from that of the stain itself. It is dependent on the ability to polymerize.

Answer 18

Metachromatic staining

Question 19

Application of a different color or stain to provide contrast and background to the staining of the structural components to be demonstrated.

Answer 19

Counterstaining