Staining Flashcards
1
Q
Gram stain steps? (4)
A
- Crystal violet
- Iodine
- Ethanol
- Safranin
2
Q
Spore stain (4)
A
- Smear it on glass
- Heat fix it
- Staining with malachite green
- Counter stain with safranin
3
Q
Acid fast stain (4)
A
- Heat fix sample
- Steaming with carbol fusion
- Acid alcohol
- Counterstain with methylene blue
4
Q
Negative stain steps (5)
A
- Droplet of dye
- Mix the bacteria into dye
- Smear it with a clean slide
- Dip into 2nd solution
- Rinse with water
5
Q
What is the purpose of gram staining?
A
To see the thickness of the peptidoglycan wall
6
Q
What is gram stain purple mean?
A
Positive & thick
7
Q
What is gram stain pink mean?
A
Negative & thin wall
8
Q
What are we looking for in spore staining?
A
Endospores
9
Q
What is a positive reaction in spore staining?
A
Green
10
Q
What is a negative reaction in spore staining?
A
Red
11
Q
What is a acid fast positive ?
A
Red
12
Q
What is a acid fast negative stain?
A
Blue
13
Q
What are we doing in a negative stain?
A
Trying to not destroy the capsule of the cell
14
Q
What is something we don’t do in negative staining and why?
A
Heat fix cause it’ll destroy the capsule we need
