Staining Flashcards
a. The name of the three-colour stains which identify the connective tissue.
b. Colours.
a. Masson’s trichrome and Mallory’s trichrome.
b. Green and blue.
Weigerts stain:
Highlight elastic fibres - dark blue or purple.
The name of the coloration which was used:
Massons trichrome and Mallorys trichrome.
The name of the coloration which was used:
Orcein elastic stain - elastic fibers appear brown.
Silver nitrate stain is used:
To identify:
a) the reticular fibers in connective tissue
b) neurofibrils in neurons
c) intercellular cement in epithelia
(brown/ black)
Stained with:
Silver nitrate
Sudan III or Sudan black
Used to stain lipids.
The name of the staining is:
Sudan III or Sudan black
- for lipids.
PAS (Periodic Acid Schiff) stain
Used to stain carbohydrates in pink (magenta)
- basement membrane.
The coloration is:
(PAS) Periodic Acid Schiff
- stains carbohydrates in pink (meagenta)
- basement membrane.
Iron hematoxylin
stains the muscle cells black
Iron hematoxylin
- stains the muscle cells black
The eight steps obtaining the histological section:
- Obtaining the tissue
- Fixation
- Dehydration
- Embedding
- Sectioning
- Staining
- Mounting
- Labeling
