Spliceosomal assembly and catalysis

Question 1

What do non-coding RNA typically do?

Answer 1

function as adaptors that position nucleic-acid targets adjacent to an enzymatic activity that is catalysed either by the RNAs themselves or by associated proteins

Question 2

Where do spliceosomal snRNA bind to on mRNA?

Answer 2

short conserved motifs located at the junctions between the expressed exon sequences and the intervening introns of target mRNAs

Question 3

Where is the 5’ splice site located on pre-mRNA?

Answer 3

At the begining of an intron

Question 4

Where is the 3’ splice site loacted on pre-mRNA?

Answer 4

At the end of an intron

Question 5

Where is the branch point adenosine usually located?

Answer 5

about 15-50 nucleotides upstream of the 3’ splice site

Question 6

What are cis-acting regulatory elements?

Answer 6

promoters, enhancers, and silencers. Regions of non-coding DNA, which regulate the transcription of nearby genes

Question 7

What do cis-acting regulatory elements do?

Answer 7

recruit trans-acting factors

Question 8

What do trans-acting factors do?

Answer 8

Control higher order particle assembly