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CHEM 211 > Spectroscopy Parts 3 and 4 > Flashcards

Spectroscopy Parts 3 and 4 Flashcards

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1
Q

What is the correct order of the parts of a single-beam instrument?

A

Light source → λ selector → Sample cell →Photo-detector → Electronics, computers

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2
Q

What emits radiation in the visible light source: Tungsten-halogen lamp?

A

Visible and NIR radiation (ca. 350-2500 nm)

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3
Q

What emits radiation in the UV light source: Deuterium lamp?

A

Mostly UV radiation

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4
Q

What is the most commonly used spectrophotometric components for variable wavelength selection?

A

Reflection gratings

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5
Q

What is the difference between constructive and destructive interference?

A

Constructive interference: when two wavelengths are added and they form a more intense wave
Destructive interference: when two wavelengths are added and they produce a single line

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6
Q

What is the order of the indispensable components of a monochromator?

A

slit → grating → slit

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7
Q

What determines the monochromator bandwidth?

A

Entrance and exit slit widths

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8
Q

Why do we want a narrow bandwidth?

A

To see spectrodetails

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9
Q

Define Dispersion

A

The angular spread of diffracted wavelengths increases as the groove density (lines per millimeter) increases. Resolution increases, working range decreases

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10
Q

What do double monochromators do?

A

Reduce stray light by orders of magnitude

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11
Q

What is the purpose of the slit, sine bar, mirror, grating?

A
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12
Q

What is the basic layout for double monochromators?

A

Entrance slit → Grating → Slit → Grating → Exit slit

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13
Q

What are the two important points for Cuvettes and Spectrophotometer cells?

A

Must provide a defined and reproducible path length and must be transparent to wavelength range of interest

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14
Q

What are microtiter plates?

A

Plates that give fast and high-throughput analysis

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15
Q

What is PMT?

A

Photomultiplier tube

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16
Q

What is the photoelectric effect?

A

Photon incident on alkali metal or metal oxide photocathode ejects an electron

17
Q

What happens in a PMT?

A

Signal amplification through an electron cascade across a series of dynodes

18
Q

True or False: Dynode, Sensitivity, Voltage, and Gains are proportional to each other

A

True

19
Q

What is the PN-junction in a Silicon Photodiode?

A

P-type material: electron acceptor (positive holes) and N-type material: electron donor

CHEM 211 (5 decks)

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