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Bio-Analytical Chemistry > Spectroscopy > Flashcards

Spectroscopy Flashcards

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1
Q

What is spectroscopy?

A

The study of the interaction between matter and electromagnetic radiation.

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2
Q

What is spectrometry?

A

The measurement of the wavelength and intensity of electromagnetic radiation.

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3
Q

What is spectrophotometry?

A

A technique used to measure how much a substance absorbs or transmits light at different wavelengths.

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4
Q

True or False: Spectroscopy can be used to identify the composition of a sample.

A

True

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5
Q

What is the unit of measurement for wavelength in spectroscopy?

A

Nanometers (nm)

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6
Q

What does UV-Vis spectroscopy measure?

A

The absorption of ultraviolet-visible light by a sample.

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7
Q

What does IR spectroscopy measure?

A

The absorption of infrared radiation by a sample.

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8
Q

What is the purpose of mass spectrometry?

A

To determine the molecular weight and structure of a compound.

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9
Q

What is the Beer-Lambert Law used for in spectrophotometry?

A

To relate the concentration of a substance in a solution to the absorbance of light by that solution.

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10
Q

What is the difference between atomic absorption spectroscopy and atomic emission spectroscopy?

A

Atomic absorption spectroscopy measures the absorption of light by free atoms, while atomic emission spectroscopy measures the emission of light by excited atoms.

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11
Q

What type of information can be obtained from a NMR spectrum?

A

Information about the number and types of atoms in a molecule, as well as their connectivity.

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12
Q

What is the purpose of fluorescence spectroscopy?

A

To study the emission of light by a substance that has absorbed light or other electromagnetic radiation.

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13
Q

What is Raman spectroscopy used for?

A

To provide information about vibrational modes in a molecule.

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14
Q

What is the difference between absorption and emission spectroscopy?

A

Absorption spectroscopy measures the absorption of light by a sample, while emission spectroscopy measures the emission of light by a sample.

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15
Q

What is the range of wavelengths for UV-Vis spectroscopy?

A

190-800 nm

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16
Q

What type of information can be obtained from a UV-Vis spectrum?

A

Information about the electronic transitions in a molecule.

17
Q

What is the purpose of X-ray spectroscopy?

A

To study the electronic structure of materials by analyzing the interaction of X-rays with the material.

18
Q

What is the difference between fluorescence and phosphorescence spectroscopy?

A

Fluorescence spectroscopy measures the immediate emission of light after absorption, while phosphorescence spectroscopy measures the delayed emission of light after absorption.

19
Q

What is the main advantage of using FT-IR spectroscopy over conventional IR spectroscopy?

A

FT-IR spectroscopy provides higher sensitivity and faster data acquisition.

20
Q

What is the purpose of flame photometry?

A

To measure the concentration of certain metal ions in a solution by measuring the intensity of light emitted by the metal ions in a flame.

21
Q

What is the difference between UV spectroscopy and visible spectroscopy?

A

UV spectroscopy measures light in the ultraviolet range (190-400 nm), while visible spectroscopy measures light in the visible range (400-800 nm).

22
Q

What is the difference between absorption and transmission spectroscopy?

A

Absorption spectroscopy measures the amount of light absorbed by a sample, while transmission spectroscopy measures the amount of light transmitted through a sample.

23
Q

What is the main advantage of using mass spectrometry for analysis?

A

High sensitivity and specificity in detecting and identifying compounds.

24
Q

What is the difference between atomic absorption spectroscopy and atomic fluorescence spectroscopy?

A

Atomic absorption spectroscopy measures the absorption of light by free atoms, while atomic fluorescence spectroscopy measures the emission of light by excited atoms.

25
Q

What is the role of a monochromator in spectrophotometry?

A

To isolate a specific wavelength of light for analysis.

26
Q

What is the main application of Raman spectroscopy in pharmaceutical analysis?

A

To identify and characterize different polymorphs of a drug substance.

27
Q

What is the difference between FT-IR and FT-NMR spectroscopy?

A

FT-IR spectroscopy analyzes the infrared region, while FT-NMR spectroscopy analyzes the nuclear magnetic resonance region.

28
Q

What is the main advantage of using HPLC coupled with UV detection in pharmaceutical analysis?

A

High resolution and sensitivity for separating and detecting compounds in a sample.

Bio-Analytical Chemistry (1 decks)

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