Spectroscopy Flashcards
What is spectroscopy?
The study of the interaction between matter and electromagnetic radiation.
What is spectrometry?
The measurement of the wavelength and intensity of electromagnetic radiation.
What is spectrophotometry?
A technique used to measure how much a substance absorbs or transmits light at different wavelengths.
True or False: Spectroscopy can be used to identify the composition of a sample.
True
What is the unit of measurement for wavelength in spectroscopy?
Nanometers (nm)
What does UV-Vis spectroscopy measure?
The absorption of ultraviolet-visible light by a sample.
What does IR spectroscopy measure?
The absorption of infrared radiation by a sample.
What is the purpose of mass spectrometry?
To determine the molecular weight and structure of a compound.
What is the Beer-Lambert Law used for in spectrophotometry?
To relate the concentration of a substance in a solution to the absorbance of light by that solution.
What is the difference between atomic absorption spectroscopy and atomic emission spectroscopy?
Atomic absorption spectroscopy measures the absorption of light by free atoms, while atomic emission spectroscopy measures the emission of light by excited atoms.
What type of information can be obtained from a NMR spectrum?
Information about the number and types of atoms in a molecule, as well as their connectivity.
What is the purpose of fluorescence spectroscopy?
To study the emission of light by a substance that has absorbed light or other electromagnetic radiation.
What is Raman spectroscopy used for?
To provide information about vibrational modes in a molecule.
What is the difference between absorption and emission spectroscopy?
Absorption spectroscopy measures the absorption of light by a sample, while emission spectroscopy measures the emission of light by a sample.
What is the range of wavelengths for UV-Vis spectroscopy?
190-800 nm
What type of information can be obtained from a UV-Vis spectrum?
Information about the electronic transitions in a molecule.
What is the purpose of X-ray spectroscopy?
To study the electronic structure of materials by analyzing the interaction of X-rays with the material.
What is the difference between fluorescence and phosphorescence spectroscopy?
Fluorescence spectroscopy measures the immediate emission of light after absorption, while phosphorescence spectroscopy measures the delayed emission of light after absorption.
What is the main advantage of using FT-IR spectroscopy over conventional IR spectroscopy?
FT-IR spectroscopy provides higher sensitivity and faster data acquisition.
What is the purpose of flame photometry?
To measure the concentration of certain metal ions in a solution by measuring the intensity of light emitted by the metal ions in a flame.
What is the difference between UV spectroscopy and visible spectroscopy?
UV spectroscopy measures light in the ultraviolet range (190-400 nm), while visible spectroscopy measures light in the visible range (400-800 nm).
What is the difference between absorption and transmission spectroscopy?
Absorption spectroscopy measures the amount of light absorbed by a sample, while transmission spectroscopy measures the amount of light transmitted through a sample.
What is the main advantage of using mass spectrometry for analysis?
High sensitivity and specificity in detecting and identifying compounds.
What is the difference between atomic absorption spectroscopy and atomic fluorescence spectroscopy?
Atomic absorption spectroscopy measures the absorption of light by free atoms, while atomic fluorescence spectroscopy measures the emission of light by excited atoms.
What is the role of a monochromator in spectrophotometry?
To isolate a specific wavelength of light for analysis.
What is the main application of Raman spectroscopy in pharmaceutical analysis?
To identify and characterize different polymorphs of a drug substance.
What is the difference between FT-IR and FT-NMR spectroscopy?
FT-IR spectroscopy analyzes the infrared region, while FT-NMR spectroscopy analyzes the nuclear magnetic resonance region.
What is the main advantage of using HPLC coupled with UV detection in pharmaceutical analysis?
High resolution and sensitivity for separating and detecting compounds in a sample.
