Spectroscopy

Question 1

Intensity of light emission of atoms or molecules of the analyte or absorption of EMR by the material measured

Answer 1

Spetroscopy

Question 2

_____ depends on the composition and structure of the material, whereas the value of analytic signal is proportional to the amount of the material.

Answer 2

Frequency

Question 3

In case the material is dissociated to atoms, the analysis methods are attributed to the group of ______

Answer 3

atomic analysis(atomic flurescence analysis,spectral analysis of atomic absorption,atomic emission)

Question 4

_______are classified according to the way the changes of energy of atoms and molecules cause the occurrence of analytical signal.

Answer 4

Spectral analysis

Question 5

Such methods are widely used for evaluation of contamination of biological objects by heavy metals, and other substances.

Answer 5

Atomic analysis and i guess spectral analysis

Question 6

Solar energy spectum

Answer 6

UV- 3 percent
vibile- 40 percent
infrared- 57 percent

Question 7

____impact of the EMR radiation upon the material This causes the changes of ____. And these are the methods of this group ,

Answer 7

Analytical signal

spectral analysis of molecular absorption (optical range of spectrum) Lumiscence analysis

Question 8

Electromagnetic radiation is dual in nature

what composes?

Answer 8

Wave and particles

Question 9

The distance between two nearest points of the same phase of a wave

Answer 9

Wavelength

Question 10

The distance between two nearest points of the same phase of a wave

Answer 10

Wavelength

Question 11

Wave number symbol and unit
Wavelength
Velocity of Radiation
Frequency of Radiation

Answer 11

V=cm-1
(λ)
C= Velocity of Radiation 3x10^10
u=Cycles/sec
V= u/c=1/2

Question 12

Wave number symbol and unit
Wavelength
Velocity of Radiation
Frequency of Radiation

Answer 12

V=cm-1
(λ)
C= Velocity of Radiation 3x10^10
u=Cycles/sec
V= u/c=1/2

Question 13

Speed of the wavefront-

Answer 13

velocity

Question 14

The reciprocal of wavelength(number of waves in unit length

Answer 14

Wavenumber

Question 15

The number of complete cycles or waves passing through a fixed point in a unit time.

Answer 15

Frequency

Question 16

Speed of light costant

Answer 16

C= 3x10 x10 cm/sec

Question 17

UV and visible reaction = _______
IR region= ____________
Wave number=_________
1 micrometer= _________

Answer 17

Angstrom(A) and nanometer
micrometer(um)
cm- or a reciprocal of a centimeter
1 micrometer= (10-6 m)

Question 18

Instrument which can measure the absorbance of a sample at any wave length

Answer 18

Spectrophotometer

Question 19

Used to identify unknown substances and determine their concentration
Measures electromagnetic absorption

Answer 19

Spectrophotometer

Question 20

Used to identify unknown substances and determine their concentration
Measures electromagnetic absorption

Answer 20

Spectrophotometer

Question 21

The concentration of an unknown sample can be determined. ________

Answer 21

When a light beam is incident to a sample, part is absorbed, and part is transmitted.

Question 22

________ is defined as the ratio of the transmitted intensity of light beam (l t / lo)

Answer 22

Transmittance (T)

Question 23

______ is defined as log (1/T)

As the _______ decrease the Absorbance increase

Answer 23

Absorbance (A)

Transmittance

Question 24

______ is defined as log (1/T)

As the _______ decrease the Absorbance increase

Answer 24

Absorbance (A)

Transmittance

Question 25

Absorbance is directly proportional to ______

Answer 25

concentration

Question 26

Working Principle
Five basic parts
1. Supply electromagnetic energy
2. Isolate the object of interest and eliminate the unwanted second order radiation
3. To accomodate sample solution
4. Receive the trasmitted light and convert it to electrical signal
5. To indicate absorbance or transmittance

Answer 26

1. Light source
2. Monochromator
3. Sample Compartment
4. Detector
5. Digital or analogue display

Question 27

Types of application of spectrophotometer

Answer 27

Chemical AnalysisFood Safety AnalysisBlood AnalysisDNA/RNA Conc. AnalysisResidual Pesticide AnalysisResidual Chlorine Analysis

Question 28

When solute absorbs EMR in the optical range of spectrum. For biological investigation ,based on measurement of visible light absorption by the solution.

Answer 28

Photometric methods

Question 29

______ is an electromagnetic radiation in the optical range of spectrum of wavelength from 400 to 800

Answer 29

Visible light

Question 30

Are applicable only for the analysis of colored solutions of the substances

Answer 30

Visual methods of colorimetry

Question 31

Concentration of the analyte solution in the methods of path length changing is estimatedaccording to beer’s law:

Answer 31

If the color intensity of the two solutions of the same material is the same, the ratio of the substance concentration

Question 32

Concentration of the analyte solution in the methods of path length changing is estimatedaccording to beer’s law:

Answer 32

If the color intensity of two solutions of the same material is the same, the ratio of the substance concentration in the solutions is in conversely proportional with the ratio of the path lengths.