Spectrophotometer Flashcards
1
Q
Spectrophotometer
Purpose of device
A
Measures the absorbance of a solution
2
Q
construct and use a calibration curve for quantitative analysis.
A
Plot the absorbance values (y-axis) against the concentrations of the standard solutions (x-axis) to create a calibration curve.
Use graphing software (like Excel) or graph paper to plot the data points.
3
Q
The Beer-Lambert Law
A
The Beer-Lambert Law indicates that absorbance is directly proportional to both the concentration and the path length through which the light passes.
4
Q
Formula
A
I = I x 10power-ecl
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