spect. Flashcards
what range (nm) are most experiments measured in? which wavelengths are included?
200-1000
UV, visible, IR
what is the highest and lowest energy?
highest: gamma, lowest: IR
what is quantum?
amount of enrgy conatiined in a single photon
what happens when exposed to ER?
electrons excited to higher energy levels
planks constant equation
E= E1 - E2 = hv
what happens energy wise when electrons are returned to ground state?
energy released as dissipation or emission spectrum
what is the visible light wavelength range?
400 - 800 10-9
why is white light polychromatic?
absorbs all wavelengths
what happens to transmitted light intesnity as light is absorbed?
decreases
how is absorption related to concentration/length of light path?
exponentially
absorbance equation
A = log10 (I0/I) no units
transmittance equation
I0/I = T%
beer-lambert equation
A = E C L
what lamps are used for visible and uv?
visible - tungesten filament
UV - deuterium discharge
cant run together
cuvette law
if 1cm path, E is same for all then absorbance is proportinal to conc.