spect. Flashcards

1
Q

what range (nm) are most experiments measured in? which wavelengths are included?

A

200-1000
UV, visible, IR

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2
Q

what is the highest and lowest energy?

A

highest: gamma, lowest: IR

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3
Q

what is quantum?

A

amount of enrgy conatiined in a single photon

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4
Q

what happens when exposed to ER?

A

electrons excited to higher energy levels

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5
Q

planks constant equation

A

E= E1 - E2 = hv

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5
Q

what happens energy wise when electrons are returned to ground state?

A

energy released as dissipation or emission spectrum

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6
Q

what is the visible light wavelength range?

A

400 - 800 10-9

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7
Q

why is white light polychromatic?

A

absorbs all wavelengths

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8
Q

what happens to transmitted light intesnity as light is absorbed?

A

decreases

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9
Q

how is absorption related to concentration/length of light path?

A

exponentially

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10
Q

absorbance equation

A

A = log10 (I0/I) no units

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11
Q

transmittance equation

A

I0/I = T%

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12
Q

beer-lambert equation

A

A = E C L

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13
Q

what lamps are used for visible and uv?

A

visible - tungesten filament
UV - deuterium discharge
cant run together

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14
Q

cuvette law

A

if 1cm path, E is same for all then absorbance is proportinal to conc.

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15
Q

what happens if sample is greater than calibration curve?

A

redilute, measure agaisnt conc. factor