Specimen preparation Flashcards
Software Management in the lab which maintains patient information and specimen testing data
Laboratory Information System( LIS)
Unlabeled specimens (Accept/Reject)
Reject except for invasive collections like CSF or other bodily fluids can be processed with signed waiver from the collector that assumes responsibility
Mislabeled specimen (spelling)
Resolved by a phone call to ordering location to verify except for Transfusion medicine (Sample is rejected)
If sample is mislabeled with another patient information and demographics do not match requisition (Reject/Accept)
Rejected
Incorrect preservative
Reject
Contaminated specimen
Reject
Incorrect blood tube
Consult lab protocol
Inappropriate volume of Na Cit tubes
Reject
Hemolyzed blood
Discretion of the medical lab tech just attach comment results but not rejected
Lipemic specimen
Technologist will ultracentrifuge to spin out fat particles
Icteric blood high levels of bilirubin (yellow/green to bright yellow)
Add comments and result
Necessary to separate plasma or serum from the cells of blood for testing
Centrifugation
Speed setting (RPM/RCF)
RPM revolutions per minute RCF is relative centrifugal force
SST clotting time before centrifuge?
Gold- 30 mins
Red- 30-60 mins
If clotting is incomplete it will result in?
Strands of fibrin in serum sample
Centrifuge Serum and plasma tubes for how long and RCF?
10 minutes at 1000-1300 RCF
Deliver unspun Na CIt tubes to coagulation bench and centrifuge for how long and RCF?
15 minutes at 1500
Whole blood (EDTA) lav should it be centrifuged or not?
NO hand delivered to hematology bench
How many times potassium in RBC than in serum?
25 times more potassium . Separation should occur within 2 hrs, delay will result in high K level and low sugar
Clots or fibrin strands on PST post-centrifugation indicates
Specimen was not mixed properly after collection
SST post-centrifugation leads to Free RBC and or fibrin strands is due to?
Centrifuging specimen before adequate clotting. Transfer serum to aliquot tube and re-spin
Common technique to share serum or plasma among different benches or test
Aliquoting specimens
Aliquoting specimen technique involves labeling tube Before or After aliquoting?
before aliquoting
Obtain label of aliquoting from the requisition or primary tube?
From the primary tube always
The aliquot tubes are placed in the front row or at the back of primary specimen tubes?
Aliquots are behind /back row
C&S samples for urine needs which type of container?
Sterile container with lids for microbiology
This urine specimen is collected any time without prior patient preparation
Random Specimen
Used for routine microbiological culture and sensitivity test for urine collection
Midstream Clean catch specimen MSU
Most concentrated specimen voided suitable for testing cytology
First morning specimen
A timed urine collection using a lab provided collection container and should be kept in the fridge
24-Hour or timed specimen
If preservatives will be added to the collection container by lab personnel, this needs to be done PRIOR or AFTER collection
Prior to start of collection
What are the 4 Lab professional role in a 24-hour urine collection ?
Prepare container for collection
Explain collection procedure
Receive and accession the specimen
Process the specimen for testing
Pediatric urine specimen (Sterile/not sterile) collection
Not Sterile from the bag transferred to a collection cup and sent to lab
