specimen collection - tubes/order of draw

Question 1

Order of draw

Answer 1

1. Blood cultures
2. Blue
3. Red
4. Yellow
5. Green
6. Dark green
7. Lavender
8. Grey

Question 2

Blood culture*

Answer 2

Whole blood
Used in microbiology
Additive - SPS/culture media

Question 3

Light blue top*

Answer 3

Plasma
Used in coagulation testing
Additive - sodium citrate
Blood:additive 9:1 important

Question 4

Red top*

Answer 4

Serum
Used in chemistry/blood bank
No additive

Question 5

Yellow top*
(from powerpoint)

Answer 5

Whole blood
Used in microbiology, HLA studies
Additives - SPS, ACD
(sodium polyethanol sulfonate/ acid citrate dextrose)

Question 6

Light green top*

Answer 6

Plasma (separator tube) PST
Used in routine chemistry
Additive - (lithium) heparin, polymer gel

Question 7

Dark green top*

Answer 7

Plasma
Used in chemistry
Additive - heparin (lithium or sodium)

Question 8

Lavender top*

Answer 8

Plasma (whole blood)
Used in hematology (CBC)
Additive - EDTA

Question 9

Grey top*

Answer 9

Plasma or serum
Used in chemistry
Additive - sodium fluoride

Question 10

White top

Answer 10

Plasma (preparation tube) PPT
Used in molecular diagnostics
Additive - polymer gel/EDTA

Question 11

Orange top

Answer 11

Serum
Used in STAT chemistry
Additives - thrombin/polymer gel

Question 12

Royal blue top

Answer 12

Plasma or serum
Used in toxicology
Additives - EDTA or clot activator

Question 13

Pink top

Answer 13

Plasma (whole blood)
Used in blood bank
Additives - EDTA
more specific than lavender

Question 14

Gold top
AKA yellow top from PDF

Answer 14

Serum
Serum Separator tube
Used in chemistry, toxicology, serology
Polymer gel