Specimen Collection

Question 1

Proper specimen collection

Answer 1

aseptic technique

Question 2

All specimens should be transported in leakproof sterile containers and processed ASAP

Answer 2

True

Question 3

Anaerobic transport media or anaerobic containers should be used for fungi.

Answer 3

False (should not be used)

Question 4

Some fungi can be affected by above 37C and below
10C

Answer 4

True

Question 5

Dermatophytes are not sensitive to cold temperatures.

Answer 5

False

Question 6

If delay is expected within 3h, specimen should be stored at 8C.

Answer 6

False (2h, 4C)

Question 7

Volume required for fungal culture is lesser than in bacteriology, since fluids and respiratory secretions need to be concentrated or pretreated prior to plating.

Answer 7

False (greater)

Question 8

For rejected or unacceptable specimens, the appropriate physicians should be notified.

Answer 8

True

Question 9

Select all that applies.

As required2, a requisition must accompany each specimen and must include the:

a. Patient name
b. Location or address
c. Age and sex
d. Physician name
e. Specific culture site
f. Date and time of specimen collection
g. Clinical diagnosis
h. Any special culture request

Answer 9

a b c d e f g h

Question 10

Release fungi enclosed within cells is important to maximize the recovery of fungi

Answer 10

True

Question 11

Concentrate fungal material in the specimen is not important to maximize the recovery of fungi

Answer 11

False (important)

Question 12

What are the three mucolytic agents?

Answer 12

1. N-acetyl-L-cysteine
2. 5% oxalic acid
3. Dithiothreitol (Sputolysin)

Question 13

Help to reduce or eliminate bacteria present in contaminated specimens because of the
action_________?

Answer 13

Mucolytic agents

Question 14

What specimens uses granules that should be washed & crushed; other materials should be centrifuge at 2,000g for 10min.

Answer 14

Abscess, drainage, pus, granules

Question 15

Specimens that are essential for best recovery.

Answer 15

Abscess, drainage, pus, granules

Question 16

Specimens that uses lysis in Isolator tubes and then centrifugation for 30min. at 3,000g, in a 35! fixed-angle rotor or swing bucket as a pretreatment

Answer 16

Blood, bone marrow

Question 17

Specimen used for the detection of H. capsulatum and other dimorphic fungi.

Answer 17

Blood, bone marrow

Question 18

What type of laminar airflow BSC is used for all work in mycology especially when dealing with filamentous fungi

Answer 18

Type 2

Question 19

Yeasts cultures can be handled on the bench in the same manner that bacterial cultures are routinely handled.

Answer 19

True

Question 20

Specimen that uses centrifugation at 2,000g for 10min. or membrane filtration as its pretreatment. Essential for best recovery with volumes 1mL; blood clots should be teased apart.

Answer 20

Body Fluids

Question 21

Specimen that needs to be minced or gently pushed pieces down into agar. It is essential for the recovery of dermatophytes.

Answer 21

Nails

Question 22

Specimen which uses lysis with mucolytic agents, followed by centrifugation at 2,000g for 10mins as pre treatment. It is also critical for the recovery of Pneumocystis jirovecii as very difficult to isolate in culture; improves recovery of other mycoses.

Answer 22

Respiratory secretions (BAL, sputum)

Question 23

Specimen that needs to be minced or ground in a mortar; push pieces down into agar as pretreatment. It is also essential for best recovery; for zygomycetes and other molds, mincing is best; for H. capsulatum, grinding is best.

Answer 23

Tissue

Question 24

Pretreatment for urine

Answer 24

Centrifugation at 2,000g for 10 mins

Question 25

Specimen that is essential for best recovery, particularly deep mycoses.

Answer 25

Urine