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CLIBAC (LAB) > Specimen Collecting and Handling > Flashcards

Specimen Collecting and Handling Flashcards

1
Q

accurate and useful determinations of results.

A

Proper Collection –

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2
Q

What is/are the goal of specimen collection?
A. To transport the specimens quickly in the lab
B. To process immediately to render a good result.
C. Maintain viability of organism
D. T avoid minimal contamination/ no contamination.

A

C and D

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3
Q

Contains the living organisms.

A

Specimen

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4
Q

How to ensure appropriate specimen management ?
A. Collect the specimen in the acute phase of infection before intakes of antibiotics.
B. Selec the atomic site for collection of specimen
C. Use proper technique with minimal contamination from normal flora
D. All of the above.

A

D

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5
Q

Bacteria sensitive to dessication

A

Haemophilus& Neisseria

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6
Q

What are the 3 types of specimens that commonly arrive in the lab?

A

Swabs
Tissue
Fluids

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7
Q

T or F
All specimens should be collected in a sterile state.

A

False- stool can be collected in just clean ad leak-proof containers.

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8
Q

Swab is recommendable or not?

A

It is not recommendable

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9
Q

The tip of swab can be made of what?

A

Cotton- excessive FA
Dacron
Calcium algitate

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10
Q

What are the source of specimen?

A
  • superficial body part area
  • deep communicating body area
  • deep close body area
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11
Q

Example of specimen in superficial body area
A. Skin tissue
B. Amniotic fluid
C. Both
D. Neither

A

A.

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12
Q

Swab can be used to collect specimen from ____.

A

Superficial body area

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13
Q

Example of Deep close body area.

A

brain spinal cord

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14
Q

T or F
Lesions, abscesses, and wounds should be preferably collected through needle aspirate.

A

True

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15
Q

Blood culture
Preparation:
Transport:
Volume:

A

Preparation:70% Isopropyl ROH /iodine
Transport: Vacutainer(SPS)
Volume: 5-10ml

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16
Q

Body fluids
Preparation:
Transport:
Volume:

A

Preparation: iodine
Transport: sterile screwcapped
Volume: N/A

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17
Q

CSF
Preparation:
Transport:
Volume:

A

Preparation: disinfect the skin
Transport: 3 sterile tubes
(CHEM-MI-HEM)
Volume: 2-10ml

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18
Q

EAR inner
Preparation:
Transport:

A

Preparation: Clean ear canal
w/ soap
Transport: Aspirate (sterile
screw capped)
Swab

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19
Q

EAR outer
Preparation:
Transport:

A

Preparation: Remove debris/crust in canal
Transport: swab

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20
Q

Preparation of eye conjuctiva.

A

Use separate swab w/ sterile NSS.

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21
Q

Corneal scraping
Preparation:
Tranapor:

A

Preparation: Local anesthesia
Tranapor: agar at bedside

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22
Q

Method in catching urine

A

Clean- catch Midstream

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23
Q

What is the 1st portion of urine

A

-washes contaminants from the urethra

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24
Q

What is the 2nd portion of urine?

A

more representative of that in the bladdercatheterized specimen

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25
Q

Specimen for For the diagnosis of bacterial pneumonia

A

Sputum

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26
Q

Most preferred time of sputum collection.

A

1st early in the morning

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27
Q

most difficult to collect
adequately because they are
contaminated with
oropharyngeal flora.

A

LRT

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28
Q

Specimen for For examination of
gastrointestinal pathogen

A

Stool

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29
Q

T or F
Bacterial infection- 3 specimens per day

A

True

30
Q

T or F
Parasites- 3 specimens for 10 days to detect ova or parasites

A

True

31
Q

Primary goal of transportating specimen

A

-maintain the specimen near to its orginal state as possible and no contamination.

32
Q

T or F
Specimen should be transported to the laboratory within 3o minutes, preferably within 24 hours.

A

False- 2 hourd

33
Q

Specimen should be stored in storage at what temp?

A

4°C within 24 hrs

34
Q

Type of specimen stored at 35°C incubator for 6 hours .

A

CSF

35
Q

Preservatices that maintain acurate colony count.

A

Boric acid (urine)

36
Q

T or F
Stool should be transported immediately .

A

True

37
Q

If the stool is transported more than 2 hours, what transport media should be used?

A

Cary-Blair media

38
Q

if the stool delay >48 hrs, the specimen should be frozen at what temp?

A

70°C

39
Q

Used to prevent clotting of specimens.

A

Anticoagulant

40
Q

most commonly used anticoagulant for microbiology specimens.

A

Sodium Polyanethol Sulfonate (SPS)

41
Q

Anticoaggulant that is often used for viral cultures and for isolation of
Mycobacterium spp. from blood.

A

Heparin

42
Q

Anticoaggulant that should not be used in microbiology
specimen.

A

EDTA and Citrate

43
Q

These usually contain substances that do not promote multiplication of microorganisms but ensure their preservation and are available in swab collection systems

A

Holding or Transport Media

44
Q

2 types of holding media.

A

 Stuart’s
 Amie’s (modified stuart’s medium)

45
Q

This media maintains favorable pH and prevents dehydration and self-destruction of the pathogen

A

Amie’s (modified stuart’s medium)

46
Q

Recommended for the preservation of N. gonorrhoeae and N. meningitidis

A

Amie’s (modified stuart’s medium)

47
Q

Transport media for stool and enteric pathogen

A

Cary and Blair

48
Q

What is/are bacteria not usually found in cary and blair Media?
A. H. Pylori
B. Salmonella
C. Shigella
D. Vibrio coma

A

A

49
Q

Group A strep is usually found in what type of tranaport media?

A

Dacron-tipped swab or calcium alginate

50
Q

Selenite F: Salmonella
Alkaline Peptone Water (APW):

A

Vibrio coma

51
Q

Commercial transport system
N. gonorrhoeae, B. pertussis

A

JEMBEC system

52
Q

Among specimen priority, what level is critical/invasive?

A

Level 1

53
Q

Which is/are not belong in specimen priority level 1?
A. CSF
B. Blood
C. Amniotic fluid
D. Bone

A

D

54
Q

Among specimen priority, what level isn’tnecessary to preserve?

A

Level 2

55
Q

Among specimen priority, what level is necessary to quanify?

A

Level 3

56
Q

Among specimen priority, what level is necessary to preserve?

A

4

57
Q

Which is/are not belong in specimen priority level 2?
A. Bone
B. Swab
C. Sputum
D. Feces

A

B.

58
Q

Which is/are not belong in specimen priority level 3?
A. Urine
B. Tissue
C. Catheter tip
D. Sputum

A

D.

59
Q

Which is/are not belong in specimen priority level 4?
A. CSF
B. Tissue
C. Feces
D. Urine

A

A and B

60
Q

Upon receiving a specimen , in macroscopic you need to take note except?
A. Stool consistency
B. Blood/mucus
C. Color of urine
D. Volume

A

C

61
Q

T or F
Fluid clarity/turbidity is an example of macroscopic examination.

A

True

62
Q

For anaerobic culture examine, which observation is not included?
A. Gas
B. Foul smell
C. Sulfur granules
D. Lactose content

A

D

63
Q

Which is/are not belong to the microscopic examination?
A. Determining the quantity of specimen
B. Indicate infectious processes
C. Routine culture
D. Fluid clarity

A

D

64
Q

1st streak =

A

1+ or light growth

65
Q

2nd or 3rd streak=

A

2+/3+ or moderate

66
Q

3rd/4th streak=

A

4+ or heavy

67
Q

How many quadrants are in culturing bacteria?

A

3-4 quadrant

68
Q

What quadrant should be atleast one quarter of the plate, streak lines should be close together?

A

Quadrant 1

69
Q

T or F
The loop must pass through one quadrant before proceeding to next one.

A

True

70
Q

T or F
Urine specimens are inoculated using quantitative isolation

A

True

71
Q

Most bacteria cultures are incubated at what temp?

A

35-37°C

72
Q

Phase of testing

A

Preanalytic
Analytic
Postanalytic

CLIBAC (LAB) (2 decks)

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