Special Media for Selected Organisms

Question 1

Thioglycolate medium is for?

Answer 1

Anaerobes

Question 2

Loffler’s coagulated serum medium is for?

Answer 2

Corynebacterium

It is a selective medium

Question 3

Tellurite agar (a differential medium) is for?

Answer 3

Corynebacterium

Question 4

Eosin methylene blue (D) and MacConkeys (D) are for?

Answer 4

Enteric bacteria
MacConkeys: pink colonies (fermentation produced acid making pink colonies); E. coli grown on eosin-methylene blue agar will be green with metallic sheen

Question 5

Hektoen enteric agar (D) and Xylose-lysine-deoxycholate agar are for?

Answer 5

Enteric pathogens

Question 6

TCBS (Thiosulfate Citrate Bile Salts Sucrose agar) (S) is for?

Answer 6

Vibrio cholerae

Question 7

Charcoal yeast extract agar (CYE agar) (S) with cystiene and iron is for?

Answer 7

Legionella

Question 8

Lowenstein-Jensen medium (S) is for?

Answer 8

Mycobacterium

Question 9

Chocolate agar is for?

Answer 9

Neisseria from normally sterile sites, Hemophilus

Question 10

Thayer-Martin selective medium (S) is for? What is it?

Answer 10

Neisseria from sites with normal flora
Chocolate agar supplemented with vancomycin, nystatin, and colistin (polymyxin) to inhibit the normal flora, including NONpathogenic neisseria

Question 11

The four Sisters Ella and the Cysteine Chapel

What is the mneumonic?

Answer 11

Francisella, brucella, legionella, and pasteurella need cysteine to grow. Growth requirements.

Question 12

What does the medium need for mycoplasma to grow?

Answer 12

Cholesterol, purines, pyrimadines

Question 13

What does Hemophilus (influenzae and aegypticus) need to grow?

Answer 13

Chocolate agar with factors X (protoporphyrin) and V (NAD)

Question 14

What is Bordet-Gengou (potato) agar for?

Answer 14

Bordetella pertussis (Bordet for Bordetella)

Question 15

What can you grown fungi on?

Answer 15

Sabouraud agar, Sab’s a fun guy