Special Culture Requirements

Question 0

Neisseria gonorrhea and meningitidis

Answer 0

Thayer Martin or
VPN media- Vancomycin ( inhibits gm positive) Polymyxin\colistin ( inhibits gm negative except neisseria) and Nystatin( inhibits fungi(

pwede pud chocolate agar

Question 1

H Influenzae

Answer 1

Chocolate agar with factors V (NAD+) and X( hematin)

Question 2

Bordtella Pertusis

Answer 2

Bordet Gengou(potato) agar

Question 3

Corynebacterium Diptheriae

Answer 3

Black colonies on cystine-tellurite agar
and
Loeffler medium produces a metachromatic blue red volutin granules

Question 4

Mycobacterium Tuberculosis

Answer 4

Lowenstein Jensen agar

Question 5

Mycobacterium pneumoniae

Answer 5

Eaton agar, requires cholesterol

Question 6

Lactose fermenting enterics

Answer 6

Pink colonies on MacConkey ( fermentation produces acid, turning colonies into pink)
E coli can also be grown on eosin methylene blue EMB agar as colonies with green metallic sheen

Question 7

Legionella

Answer 7

Charcoal yeast extract agar buffered with cysteine and iron

Question 8

Anaerobes

Answer 8

Thioglycolate

Question 9

V cholerae

Answer 9

TCBS thiosulfate citrate bile salt sucrose agar

Question 10

Fungi

Answer 10

Sabouraud agar