spec 1

Question 1

what does UV show about drug

Answer 1

• electron conjugation
• purity = use calibration curve
• acidity/basicity
• single/multiple bonds

Question 2

IR

Answer 2

-functional groups

Question 3

where does carbonyl absorb in IR

Answer 3

at 1700 except in amide where its slightly lower due to amide conjugation

Question 4

mass spectrometer

Answer 4

• moleuclar weight
• isomers/ fragmentation
• isotopes
• metabolite analysis

Question 5

NMR

Answer 5

which atoms, how many, connection

purity

Question 6

what is the order of increasing energy

Answer 6

UV–> Visible –> IR —> NMR

Question 7

what is the trend of wave energy

Answer 7

more energetic the wave, the bigger the frequency but the shorter wavelength

Question 8

Why can’t you see the UV absorption of some drugs

Answer 8

all absorb UV but some absorb in vacuum region of less than 200nm this absorbance can’t be seen unless air is taken away

Question 9

what does beer law show

Answer 9

light absorption by drug is directly proportional to conc of drug

Question 10

Lambert law

Answer 10

total light absorption is directly proportional to light length

Question 11

Beer-Lambert Law + units

Answer 11

A=ecl

A = absorbance
e = molar extinction coefficient (L.mol-1.cm-1)
c = drug concentration (mol.L-1)
l = path length (cm)

Question 12

factors that impact the beer-lambert law

Answer 12

precipitation
temp
photolysis