Southern blotting + denaturation

Question 1

Southern blotting + hybridization methods devised by him

Answer 1

edward southern

Question 2

detects the location of DNA + probe on the filter

Answer 2

autoradiography

Question 3

Applications of southern hybridization

Answer 3

RFLP mapping; forensic investigation; genetic screening

Question 4

steps in southern hybridization

Answer 4

restriction digestion; electrophoresis; blotting and denaturation; Hybridization with radioactive probe; Autoradiography

Question 5

DNA samples to be tested are prepared from the appropriate sources

Answer 5

restrcition digestion

Question 6

The mixtures of restriction fragments from each sample are separated by this

Answer 6

electrophoresis

Question 7

Capillary action pulls an alkaline solution upward through the gel and through a sheet of nitrocellulose paper

Answer 7

blotting and denaturation

Question 8

The paper blot is exposed to a solution containing radioactively labeled probe

Answer 8

Hybridization with radioactive probe

Question 9

A sheet of photographic film is laid over the paper

Answer 9

Autoradiography

Question 10

three other blotting methods

Answer 10

northern blotting; western blotting; eastern blotting

Question 11

RNA blotted on membrane; DNA probe

Answer 11

Northern blotting

Question 12

Proteins blotted; Labeled antibody specific for the protein

Answer 12

Western blotting

Question 13

Used to detect glycoproteins and lipoproteins

Answer 13

Eastern blotting

Question 14

verification of ZmCA1 insertion

Answer 14

PCR

Question 15

verification of protein expression

Answer 15

Western blot analysis

Question 16

quantification of carbonic anhydrase activity

Answer 16

Enzyme assay

Question 17

detection of copy number of gene insertion

Answer 17

Southern blot analysis

Question 18

Process of strand separation

Answer 18

Denaturation

Question 19

plot of temperature vs extent of denaturation

Answer 19

Melting curve

Question 20

midpoint of the temperature range over which strands of DNA separate

Answer 20

Melting temperature

Question 21

The transient denaturation; Small fraction of base pairs in the genome

Answer 21

DNA breathing

Question 22

How to denature DNA?

Answer 22

Heat; Alkali; Melting proteins

Question 23

Ability of the two separated complementary strands to reform into the ordered stated

Answer 23

Renaturation

Question 24

two steps of renaturation

Answer 24

nucleation; zippering

Question 25

DNA or RNA fragment of known sequence; Complementary to the target DNA/RNA

Answer 25

Probe

Question 26

Labeled radioactive or non radioactively; detects specific sequence nucleotide sequences in the DNA/RNA sample

Answer 26

probe