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cytology > slides > Flashcards

slides Flashcards

1
Q

simple columnar epithelium

A
  • H&E staining
  • found in wall of gallbladder- low magnification
  • the innermost layer of its wall is built up of one layer of columnar epithelial cells resting on a basement membrane.
  • the cells have one basally located nucleus stained in blue by the hematoxylin.
  • no villi
  • no crypts
  • no submucosa
  • no goblet cells
  • violet lilac colour
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2
Q

simple cuboidal epithelium

A
  • found in the thyroid gland
  • H&E staining
  • follicles first found low magnification
  • homogenous eosinophillic matter- colloid. in the middle
  • purple thyrocytes observed lining the wall of each follicle
  • each thyrocyte shas one centrally located nucleus
  • pinkish purple
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3
Q

stratified squamous keratinized epithelium

A
  • found in glabrous skin
  • azan staining
  • epidermis and underlying dermis low mag
  • various shapes found columnar polygonal squamous
  • cell nuclei stained in red
  • cytoplasm and its components different shades of red and orange
  • deep red violet blue
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4
Q

acid phosphotase

A
  • found in liver
  • method of gomori
  • considered as a marker enzyme for lysosomes
  • brownish yellow
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5
Q

succinate dehydrogenase

A
  • found in kidney
  • tetrazolium method of nachlas
  • catalyzes the oxidation of the succinate to fumarate
  • located on the inner mitochondrial membrane
  • deep dark violet colour
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6
Q

glycogen

A
  • found in liver
  • PAS reaction
  • two sections- one starved other recently fed
  • recently fed- intense magenta observed- glycogen granules in the liver cells- positive PAS reaction
  • at large mag- glycogen is not evenly distributed in cells laid down in one of the cell poles- the escape of glycogen- due to the tissue processing prior to the PAS reaction.
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7
Q

lipids

A
  • subcutaneous adipose tissue
  • Sudan III staining- lipid soluble
  • orange adipose tissue- seperated by loose connective tissue
  • round-oval cells unilocular adipocytes- seen in clusters
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8
Q

mucopolysaccharides

A
  • mucous acini in the sublingual gland
  • acian blue staining
  • electric blue surrounded by neutral red
  • mucopolysaccarides all in cytoplasm
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9
Q

simple squamous epithelium

A
  • silver impregnation
  • muddy browish orangish bubbles (pertoneum)
  • the borders, brownish webs (omentum)
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10
Q

stereocilia

A
  • epididymis
  • H&E staining
  • two cell types- tall columnar and basal cells
  • the inner ring are the stereocilia
  • pink
  • large lumen
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11
Q

brush border

A
  • small intestine
  • H&E staining
  • microvilli are 0.5 to 1μm long
  • the edge of the small intestine
  • long purple edges look like bristles of a brush
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12
Q

mitochondria

A
  • kidney
  • iron hematoxylin staining
  • dark greyish blackish
  • kidney bean looking
  • the granules at the basal cellular compartment are the mitochondria
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13
Q

golgi apparatus

A
  • spinal ganglion
  • silver impregnation
  • orange brownish
  • roundish packed with thicker darker brown borders
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14
Q

secretory vesicles

A
  • pancreas
  • iron hematoxylin staining
  • pancreatic acini at high mag- rich in secretory vesicles
  • light grey with clear tiger stripes
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15
Q

elongated nuclei

A
  • small intestine
  • H&E staining
  • found in the smooth muscle cells of the intestinal wall
  • longitudinal pink layer
  • straight cells
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16
Q

spherical nuclei

A
  • dorsal root spinal ganglion
  • H&E staining
  • pale pink
    find white circles on mag 10 then they’re everywhere
  • surrounded by satellite cells
  • purple dots small
17
Q

simple squamous epithelium in parietal layer of the Bowman’s capsule

A
  • kidney
  • H&E staining
  • circular surrounded by a clear area
  • pink in colour
18
Q

simple cuboidal covering epithelium in excretory ducts

A
  • submandibular gland
  • h&E staining
  • round shaped structures with lumens
  • pink in colour
19
Q

simple columnar secretory epithelium

A
  • stomach
  • h&E staining
  • found in the edge
  • squiggly waves- deep columns
  • purple dots inner line
20
Q

simple columnar absorptive epithelium

A
  • same as brush border - H&E staining small intestine
21
Q

pseudostratified columnar ciliated epithelium
(H&E staining)

A
  • trachea
  • thick pink border with dots
22
Q

pseudostratified columnar ciliated epithelium (masson trichrome staining)

A
  • trachea
  • aqua blue and magenta looking straight thick line clearish on the inside
23
Q

transitional epithelium

A
  • urinary bladder
  • H&E staining
  • thick purple border
  • deep purple
  • at the wall
    large squiggles on the edge
24
Q

stratified squamous non keratinized epithelium

A
  • esophagus
  • H&E staining
  • huge purple dark purple inline reverse fade thick border
  • zoom in to clear cells with purple nuclei
25
Q

stratified squamous keratinized epithelium - H&E staining

A
  • glabrous skin
  • deep magenta outline, purple fade on the inside
26
Q

simple tubular glands

A
  • pylorus of the stomach
  • H&E staining
  • clear branch in middle of light purple and pink
27
Q

compound alveolar gland- serous acini

A
  • parotid gland
  • H&E staining
  • deep pink with clear bubbles
28
Q

compound alveolar gland mucous acini

A
  • sublingual gland
  • H&E staining
  • clear circles with bubbles, purple dots outline
29
Q

compound alveolar gland mixed acini

A
  • submandibular gland
  • H&E staining
  • pink with purple dots on one side as well as clear areas
30
Q

endocrine glands

A
  • suprarenal cortex
  • H&E staining
  • pale pink space with pink dots
31
Q

mesenchyme

A
  • found in the phalanx (fetus)
  • H&E staining
  • large mag- faint lilac almost clear
  • pale lilac space with purple dots
32
Q

loose connective tissue

A
  • hypodermis
  • H&E staining
  • red webs faded
33
Q

dense regular connective tissue

A
  • tendon
  • H&E staining
  • bright pink lines with few every other purple dot
34
Q

reticular connective tissue

A
  • lymph node
  • Gomori silver impregnantion
  • intensely packed brown miniscule spots
35
Q

white unilocular adipose tissue

A
  • hypodermis
  • H&E staining
  • pale pink circles with sparce yellow dots on its border
36
Q

brown multilocular adipose connective tissue

A
  • interscapular reigon of a new born
  • H&E staining
  • brown sparse dots
37
Q

hyaline cartilage

A
  • trachea
  • H&E staining
  • pink space with a few small holes and deep pink lines every now and then

cytology (4 decks)

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