slides Flashcards
1
Q
simple columnar epithelium
A
- H&E staining
- found in wall of gallbladder- low magnification
- the innermost layer of its wall is built up of one layer of columnar epithelial cells resting on a basement membrane.
- the cells have one basally located nucleus stained in blue by the hematoxylin.
- no villi
- no crypts
- no submucosa
- no goblet cells
- violet lilac colour
2
Q
simple cuboidal epithelium
A
- found in the thyroid gland
- H&E staining
- follicles first found low magnification
- homogenous eosinophillic matter- colloid. in the middle
- purple thyrocytes observed lining the wall of each follicle
- each thyrocyte shas one centrally located nucleus
- pinkish purple
3
Q
stratified squamous keratinized epithelium
A
- found in glabrous skin
- azan staining
- epidermis and underlying dermis low mag
- various shapes found columnar polygonal squamous
- cell nuclei stained in red
- cytoplasm and its components different shades of red and orange
- deep red violet blue
4
Q
acid phosphotase
A
- found in liver
- method of gomori
- considered as a marker enzyme for lysosomes
- brownish yellow
5
Q
succinate dehydrogenase
A
- found in kidney
- tetrazolium method of nachlas
- catalyzes the oxidation of the succinate to fumarate
- located on the inner mitochondrial membrane
- deep dark violet colour
6
Q
glycogen
A
- found in liver
- PAS reaction
- two sections- one starved other recently fed
- recently fed- intense magenta observed- glycogen granules in the liver cells- positive PAS reaction
- at large mag- glycogen is not evenly distributed in cells laid down in one of the cell poles- the escape of glycogen- due to the tissue processing prior to the PAS reaction.
7
Q
lipids
A
- subcutaneous adipose tissue
- Sudan III staining- lipid soluble
- orange adipose tissue- seperated by loose connective tissue
- round-oval cells unilocular adipocytes- seen in clusters
8
Q
mucopolysaccharides
A
- mucous acini in the sublingual gland
- acian blue staining
- electric blue surrounded by neutral red
- mucopolysaccarides all in cytoplasm
9
Q
simple squamous epithelium
A
- silver impregnation
- muddy browish orangish bubbles (pertoneum)
- the borders, brownish webs (omentum)
10
Q
stereocilia
A
- epididymis
- H&E staining
- two cell types- tall columnar and basal cells
- the inner ring are the stereocilia
- pink
- large lumen
11
Q
brush border
A
- small intestine
- H&E staining
- microvilli are 0.5 to 1μm long
- the edge of the small intestine
- long purple edges look like bristles of a brush
12
Q
mitochondria
A
- kidney
- iron hematoxylin staining
- dark greyish blackish
- kidney bean looking
- the granules at the basal cellular compartment are the mitochondria
13
Q
golgi apparatus
A
- spinal ganglion
- silver impregnation
- orange brownish
- roundish packed with thicker darker brown borders
14
Q
secretory vesicles
A
- pancreas
- iron hematoxylin staining
- pancreatic acini at high mag- rich in secretory vesicles
- light grey with clear tiger stripes
15
Q
elongated nuclei
A
- small intestine
- H&E staining
- found in the smooth muscle cells of the intestinal wall
- longitudinal pink layer
- straight cells
16
Q
spherical nuclei
A
- dorsal root spinal ganglion
- H&E staining
- pale pink
find white circles on mag 10 then they’re everywhere - surrounded by satellite cells
- purple dots small
17
Q
simple squamous epithelium in parietal layer of the Bowman’s capsule
A
- kidney
- H&E staining
- circular surrounded by a clear area
- pink in colour
18
Q
simple cuboidal covering epithelium in excretory ducts
A
- submandibular gland
- h&E staining
- round shaped structures with lumens
- pink in colour
19
Q
simple columnar secretory epithelium
A
- stomach
- h&E staining
- found in the edge
- squiggly waves- deep columns
- purple dots inner line
20
Q
simple columnar absorptive epithelium
A
- same as brush border - H&E staining small intestine
21
Q
pseudostratified columnar ciliated epithelium
(H&E staining)
A
- trachea
- thick pink border with dots
22
Q
pseudostratified columnar ciliated epithelium (masson trichrome staining)
A
- trachea
- aqua blue and magenta looking straight thick line clearish on the inside
23
Q
transitional epithelium
A
- urinary bladder
- H&E staining
- thick purple border
- deep purple
- at the wall
large squiggles on the edge
24
Q
stratified squamous non keratinized epithelium
A
- esophagus
- H&E staining
- huge purple dark purple inline reverse fade thick border
- zoom in to clear cells with purple nuclei
25
Q
stratified squamous keratinized epithelium - H&E staining
A
- glabrous skin
- deep magenta outline, purple fade on the inside
26
Q
simple tubular glands
A
- pylorus of the stomach
- H&E staining
- clear branch in middle of light purple and pink
27
Q
compound alveolar gland- serous acini
A
- parotid gland
- H&E staining
- deep pink with clear bubbles
28
Q
compound alveolar gland mucous acini
A
- sublingual gland
- H&E staining
- clear circles with bubbles, purple dots outline
29
Q
compound alveolar gland mixed acini
A
- submandibular gland
- H&E staining
- pink with purple dots on one side as well as clear areas
30
Q
endocrine glands
A
- suprarenal cortex
- H&E staining
- pale pink space with pink dots
31
Q
mesenchyme
A
- found in the phalanx (fetus)
- H&E staining
- large mag- faint lilac almost clear
- pale lilac space with purple dots
32
Q
loose connective tissue
A
- hypodermis
- H&E staining
- red webs faded
33
Q
dense regular connective tissue
A
- tendon
- H&E staining
- bright pink lines with few every other purple dot
34
Q
reticular connective tissue
A
- lymph node
- Gomori silver impregnantion
- intensely packed brown miniscule spots
35
Q
white unilocular adipose tissue
A
- hypodermis
- H&E staining
- pale pink circles with sparce yellow dots on its border
36
Q
brown multilocular adipose connective tissue
A
- interscapular reigon of a new born
- H&E staining
- brown sparse dots
37
Q
hyaline cartilage
A
- trachea
- H&E staining
- pink space with a few small holes and deep pink lines every now and then
