Single Cell RNA Seq

Question 1

Technical duplicates in the context of single-cell sequencing arise when
(Single choice)
a) Two cells form a discrete unit that does not break apart during
disruption to form a suspension.
b) Two or more cells are captured in a droplet with a single bead.
c) Two or more cells are separately encapsulated with beads that contain
identical barcodes.
d) One cell is captured in a droplet with more than one bead

Answer 1

c) Two or more cells are separately encapsulated with beads that contain
identical barcodes.

Question 2

Suppose that you are given a single-cell RNAseq data set and you need to do
dimensionality reduction to cluster and annotate cell types later. Given that
you want to preserve both local and global structures, which of the methods
below is the preferred dimensionality reduction approach to be used in this
case (Single choice):
a) t-SNE
b) PCA
c) UMAP

Answer 2

c) UMAP