SFP21: Methods To Study Protein-protein Interactions Flashcards
1
Q
Name 3 methods to identify protein-protein interactions
A
- yeast 2-hybrid screening
- affinity chromatography
- Immunoprecipitation (technically a form of affinity chromatography)
2
Q
What is LC-MS/MS?
A
- generate tryptic peptides exactly as for PMF or MS/MS but of protein mixtures
- Peptide mixtures separated by liquid chromatography (LC)
- 1000s of individually peptides from the mixture rapidly and equitably sequenced by MS/MS
