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Microscope > Setup/harvest > Flashcards

Setup/harvest Flashcards

1
Q

Antibiotics

A

Penicillin/streptomycin

Kanamycin
Gentamicin

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2
Q

Antifungicides

A

Amphotercin B

Fungizone

Mycostatin (Nystatin)

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3
Q

Amniotic fluid gestation time

A

Ideal gestation is 16-20 weeks

Can be as early as 12 weeks

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4
Q

Basic media

A

Contains the minimum ingredients necessary for cells to grow

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5
Q

Modified media

A

Contains a specific mix of nutrients necessary for a specific type of cell

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6
Q

Enriched media

A

A basic media that contains extra amino acids, vitamins, minerals

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7
Q

Complete media

A

Contains all the required nutrients plus supplements such as 10-20% fetal bovine serum, L-glutamine, and antibiotics

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8
Q

Maintenance media

A

Contains minimal serum (1-3%)

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9
Q

Conditioned media

A

Contains growth factors and other factors secreted by growing cells. Giant cell tumor conditioned media can be used to enhance the growth of leukemic cells

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10
Q

L-glutamine

A

Essential amino acid
Unstable

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11
Q

Cell cycle synchronization

A

Blocks cells in the s phase

Methotrexate (MTX) aka amethopterin blocks the action of thymidylate synthetase

FUdR 5-fluorodeoyuridine

Excess thymidine or BrdU

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12
Q

Trypsin

A

Commonly used to remove cells from flasks

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13
Q

Cell cycle

A

Interphase
Prophase
Metaphase
Anaphase
Telophase
Cytokinesis

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14
Q

Fixative

A

Kills the cells
Fixes the membrane
Precipitates proteins and removes the H1 fraction of the histone proteins

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15
Q

Age slides

A

Overnight at 60
2 hours at 75
20 minutes at 90

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16
Q

Humidity and temp for slide dropping

A

50-60%

24 to 27

17
Q

Slide conditions

Hot & wet

A

Evaporation too slow (overspreading)

Drop on dry slides
Try warm slides
Wave slides
Increase methanol

18
Q

Slide conditions

Hot & dry

A

Evaporation too fast (underspreading)

Drop on wet slides
Dry slides on wet paper towel or humidifier
Try cold slides
Increase acetic acid

19
Q

Late replicating DNA

A

Dark G and Q bands
C bands
Light r bands
Positive heteropyknoesis
Heterochromatin
AT rich

20
Q

Early Replicating DNA

A

Light G and Q bands
Dark r bands
Negative heteropyknoesis
Euchromatin
GC rich

21
Q

Positive heteropyknoesis

A

Chromosome regions that have few genes and contain primarily noncoding regions of DNA

More contracted or supercoiled

Microscope (4 decks)

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