Session One Flashcards
What prevents tissues from rotting
Formalin
What do you use to embed tissue in to allow it be sliced thinly ?
Melted paraffin that sets hard when cooled
Two types of stains
Haemotoxylin and Eosin
This stain is blue and stains the nucleus strongly
Haemotoxylin
This stain is pink and stains the cytoplasm and ECM strongly
Eosin
How do you prepares Frozen section
- Freeze surgical specimen immediately at -20 to -30
- Use microtome part of the cryostat ( microtome in freezer) to cut the specimen into thin pieces
- Stain using eosin and haemotoxylin
Normal body temperature range ?
36.1 - 37.2
Core temperature is ?
Temperature of the internal environment
Hypothermia occurs below what temperature ?
35 degrees
What are monoclonal antibodies
Antibodies that are made by identical immune cells that are all clones of a unique parent cell
What are humanised monoclonal antibodies ?
Human antibody plus rat monoclonal antibody which does the binding to the antigen
Integumentary means ?
Protect the body from various kinds of damage
Difference between Gout and Pseudogout ?
Gout is usually in the big toe and knee ; it is an accumulation of monosodium urate monohydrate crystals.
Pseudogout is usually in the knee and is an accumulation of calcium pyrophosphate dihydrate crystals
Confocal microscopy resembles the imaging process in computed axial tomography scanning ?
True
What is immunohistochemistry
It is the process of detecting antigens in cells of a tissue section by exploiting the fact that antibodies bind to specific antigens