session 8-molecular diagnosis

Question 1

see notes on penultimate

Answer 1

see notes on penultimate

Question 2

Bacteria produce……to cut specific DNA sequences and act as restriction enzymes.

Answer 2

Endonucleases

Question 3

Give the name of the technique you use to analyse DNA at the nucleotide level.

Answer 3

DNA sequencing

Question 4

Give the names of the 4 methods used to analyse DNA at the GENE level.

Answer 4

Southern blotting
RT-PCR
Microarray
DNA fingerprinting

Question 5

What are the names of the 2 methods used to analyse DNA at the CHROMOSOME level?

Answer 5

Karyotyping

FISH

Question 6

Give 4 reasons why you would use fish.

Answer 6

-investigate:
Genes
Chromosome structure
Chromosome number
Chromosome behaviour