Serology/Molecular

Question 1

Which antibodies will R1 adsorbing cells adsorb?

Answer 1

Alllo or auto-anti-D, C, e

Question 2

What antibodies will an R2 adsorbing cell adsorb?

Answer 2

Allo or auto-anti-D,-c,-E

Question 3

What antibodies will rr adsorbing cells adsorb?

Answer 3

Allo or auto-ant-c-e-f

Question 4

What is the unifying hypothesis related to drug induced hemolytic anemia?

Answer 4

Comprehensive approach recognizing that DIHA may be caused by multiple mechanisms:
Antibody to drug
Antibody to the red cell membrane components
Antibody to the drug and membrane components
NIPA (Non-immunologic protein adsorption

Question 5

Drug dependent antibodies divided into two categories

Answer 5

1. Those that react with drug treated red cells (I.e. penicillin and some cephalosporins)
2. Those that react with untreated red cells in the presence of a solution of the drug (quinine and ceftriaxone)

Question 6

Drug-independent antibodies characteristics

Answer 6

1. serologic reactivity is independent of the drug despite the fact that the drug originally induced the immune response
2. behave like autoantibodies/serologically indistinguishable from idiopathic WAA

Question 7

What are the most common drugs associated with Drug-Independent Autoantibody?

Answer 7

Methyldopa (not used as frequently)
Fludarabine (used to treat CLL)

Question 8

Which antigens are destroyed by proteases?

Answer 8

M, N, Fya, Fyb,LW, YT, Dombrock, Knops