Serology Flashcards
What is the difference between specificity and sensitivity?
Specificity: false positives
Sensitivity: false negatives
What do most serologic tests require as a sample?
Serum and plasma
What does ELISA stand for?
Enzyme-linked immunosorbent assay
What does CELISA stand for?
Competitive enzyme-linked immunosorbent assay
What Latex Agglutination tests, what does a positive result look like?
Agglutination
RIM is also known as?
Rapid Immunomigration
How does a positive tests for RIM present?
Two areas of color, test, & control
What does a Coomb’s test evaluate?
Antibodies against the body
What does a positive immunodiffusion test look like?
Band of precipitation
What indicates a positive IFA test?
Agglutination
What two times are samples collected for antibody titers?
Acute Phase (when animal is showing clinical signs) Convalescent Phase (2-6 weeks later)
A positive Ab titer is when there is a ___ -fold ___ in titer levels
4; rise
A change in titer is called a ___.
Seroconversion
High titer indicates?
Active infection
Low titer indicates?
Previous exposure
Molecular diagnostic tests analyze ___ or ___.
DNA or RNA
What are 3 types of molecular diagnostic tests?
PCR, Reverse Transcriptase PCR, Real-Time PCR
A positive ID allergy test shows what reaction?
Raised welt
A Tb skin test is testing for which microorganism?
Mycobacterium spp.
In FeLV what is the time from infection until presence of detectable Ag?
Within 28 days
Is FeLV an Ab or Ag test?
Ag
In FeLV will the vaccine cause the test to be positive?
No
What is FeLV confirmation test?
IFA
In FIV what is the time from infection until presence of detectable Ab?
Within 60 days