Serology Flashcards
What is the difference between specificity and sensitivity?
Specificity: false positives
Sensitivity: false negatives
What do most serologic tests require as a sample?
Serum and plasma
What does ELISA stand for?
Enzyme-linked immunosorbent assay
What does CELISA stand for?
Competitive enzyme-linked immunosorbent assay
What Latex Agglutination tests, what does a positive result look like?
Agglutination
RIM is also known as?
Rapid Immunomigration
How does a positive tests for RIM present?
Two areas of color, test, & control
What does a Coomb’s test evaluate?
Antibodies against the body
What does a positive immunodiffusion test look like?
Band of precipitation
What indicates a positive IFA test?
Agglutination
What two times are samples collected for antibody titers?
Acute Phase (when animal is showing clinical signs) Convalescent Phase (2-6 weeks later)
A positive Ab titer is when there is a ___ -fold ___ in titer levels
4; rise
A change in titer is called a ___.
Seroconversion
High titer indicates?
Active infection
Low titer indicates?
Previous exposure
Molecular diagnostic tests analyze ___ or ___.
DNA or RNA
What are 3 types of molecular diagnostic tests?
PCR, Reverse Transcriptase PCR, Real-Time PCR
A positive ID allergy test shows what reaction?
Raised welt
A Tb skin test is testing for which microorganism?
Mycobacterium spp.
In FeLV what is the time from infection until presence of detectable Ag?
Within 28 days
Is FeLV an Ab or Ag test?
Ag
In FeLV will the vaccine cause the test to be positive?
No
What is FeLV confirmation test?
IFA
In FIV what is the time from infection until presence of detectable Ab?
Within 60 days
Is FIV an Ab or Ag test?
Ab
Will FIV’s vaccine cause the test to be positive?
Yes
What is FIV’s confirmation test?
Western Blot
In FIV when should positive kittens be retested?
6 months old
In FIV why can there be false positives?
Maternal derived Ab’s
In Parvo how long does fecal shedding occur? How long after exposure?
7-10 days; 3-5 days
Is Parvo an Ab or Ag test?
Ag
Is Lyme Disease an Ag or Ab test?
Ab
Detectable levels in Lyme Disease may not be present for how long post exposure?
4-6 weeks
Will Lyme Disease’s vaccine cause a positive result?
No
Is Ehrlichia an Ag or Ab test?
Ab
Ehrlichia can also be diagnosed by?
Modulation in WBC’s
Brucellosis is what type of test?
Rapid Slide Agglutination Test
Is Brucellosis an Ag or Ab test?
Ab
Is Heartworm in dogs an Ag or Ab test?
Ag
When is heartworm in dogs first detectable?
5 months post infection
How long after treatment in heartworm in dogs should Ag be undetectable?
4-5 months
Heartworm-cat (Ab)
As early as ___ post-infection
2 months
Heartworm-cat (Ag)
Only picks up Ag to which gender of worms?
Female
Heartworm-cat
What is the best diagnostic method?
Echocardiogram
Canine pregnancy- Relaxin
Produced by?
Placenta
Canine pregnancy- Relaxin
How long following fertilization?
22-25 days
Canine pregnancy- Relaxin
Levels peak around?
40-50 days
Canine pregnancy- Relaxin
False negatives?
Small litters, one of more dead puppies
Canine ovulation- Luteinizing Hormone
Bitch is fertile ___ following the LH surge
4-7 days
Canine ovulation- Luteinizing Hormone
How often is the test ran?
Daily
Canine ovulation- Progesterone
How often is the test ran?
Every 2-3 days
Why is the foal/calf Ig test ran?
Useful in preventing disease related to failure of passive transfer of IgG from colostrum
