Serology Flashcards

1
Q

What is the difference between specificity and sensitivity?

A

Specificity: false positives
Sensitivity: false negatives

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2
Q

What do most serologic tests require as a sample?

A

Serum and plasma

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3
Q

What does ELISA stand for?

A

Enzyme-linked immunosorbent assay

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4
Q

What does CELISA stand for?

A

Competitive enzyme-linked immunosorbent assay

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5
Q

What Latex Agglutination tests, what does a positive result look like?

A

Agglutination

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6
Q

RIM is also known as?

A

Rapid Immunomigration

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7
Q

How does a positive tests for RIM present?

A

Two areas of color, test, & control

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8
Q

What does a Coomb’s test evaluate?

A

Antibodies against the body

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9
Q

What does a positive immunodiffusion test look like?

A

Band of precipitation

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10
Q

What indicates a positive IFA test?

A

Agglutination

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11
Q

What two times are samples collected for antibody titers?

A
Acute Phase (when animal is showing clinical signs)
Convalescent Phase (2-6 weeks later)
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12
Q

A positive Ab titer is when there is a ___ -fold ___ in titer levels

A

4; rise

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13
Q

A change in titer is called a ___.

A

Seroconversion

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14
Q

High titer indicates?

A

Active infection

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15
Q

Low titer indicates?

A

Previous exposure

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16
Q

Molecular diagnostic tests analyze ___ or ___.

A

DNA or RNA

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17
Q

What are 3 types of molecular diagnostic tests?

A

PCR, Reverse Transcriptase PCR, Real-Time PCR

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18
Q

A positive ID allergy test shows what reaction?

A

Raised welt

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19
Q

A Tb skin test is testing for which microorganism?

A

Mycobacterium spp.

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20
Q

In FeLV what is the time from infection until presence of detectable Ag?

A

Within 28 days

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21
Q

Is FeLV an Ab or Ag test?

22
Q

In FeLV will the vaccine cause the test to be positive?

23
Q

What is FeLV confirmation test?

24
Q

In FIV what is the time from infection until presence of detectable Ab?

A

Within 60 days

25
Is FIV an Ab or Ag test?
Ab
26
Will FIV's vaccine cause the test to be positive?
Yes
27
What is FIV's confirmation test?
Western Blot
28
In FIV when should positive kittens be retested?
6 months old
29
In FIV why can there be false positives?
Maternal derived Ab's
30
In Parvo how long does fecal shedding occur? How long after exposure?
7-10 days; 3-5 days
31
Is Parvo an Ab or Ag test?
Ag
32
Is Lyme Disease an Ag or Ab test?
Ab
33
Detectable levels in Lyme Disease may not be present for how long post exposure?
4-6 weeks
34
Will Lyme Disease's vaccine cause a positive result?
No
35
Is Ehrlichia an Ag or Ab test?
Ab
36
Ehrlichia can also be diagnosed by?
Modulation in WBC's
37
Brucellosis is what type of test?
Rapid Slide Agglutination Test
38
Is Brucellosis an Ag or Ab test?
Ab
39
Is Heartworm in dogs an Ag or Ab test?
Ag
40
When is heartworm in dogs first detectable?
5 months post infection
41
How long after treatment in heartworm in dogs should Ag be undetectable?
4-5 months
42
Heartworm-cat (Ab) As early as ___ post-infection
2 months
43
Heartworm-cat (Ag) Only picks up Ag to which gender of worms?
Female
44
Heartworm-cat What is the best diagnostic method?
Echocardiogram
45
Canine pregnancy- Relaxin Produced by?
Placenta
46
Canine pregnancy- Relaxin How long following fertilization?
22-25 days
47
Canine pregnancy- Relaxin Levels peak around?
40-50 days
48
Canine pregnancy- Relaxin False negatives?
Small litters, one of more dead puppies
49
Canine ovulation- Luteinizing Hormone Bitch is fertile ___ following the LH surge
4-7 days
50
Canine ovulation- Luteinizing Hormone How often is the test ran?
Daily
51
Canine ovulation- Progesterone How often is the test ran?
Every 2-3 days
52
Why is the foal/calf Ig test ran?
Useful in preventing disease related to failure of passive transfer of IgG from colostrum