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Clin Lab III > Serology > Flashcards

Serology Flashcards

1
Q

What is the difference between specificity and sensitivity?

A

Specificity: false positives
Sensitivity: false negatives

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2
Q

What do most serologic tests require as a sample?

A

Serum and plasma

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3
Q

What does ELISA stand for?

A

Enzyme-linked immunosorbent assay

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4
Q

What does CELISA stand for?

A

Competitive enzyme-linked immunosorbent assay

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5
Q

What Latex Agglutination tests, what does a positive result look like?

A

Agglutination

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6
Q

RIM is also known as?

A

Rapid Immunomigration

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7
Q

How does a positive tests for RIM present?

A

Two areas of color, test, & control

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8
Q

What does a Coomb’s test evaluate?

A

Antibodies against the body

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9
Q

What does a positive immunodiffusion test look like?

A

Band of precipitation

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10
Q

What indicates a positive IFA test?

A

Agglutination

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11
Q

What two times are samples collected for antibody titers?

A 
Acute Phase (when animal is showing clinical signs)
Convalescent Phase (2-6 weeks later)
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12
Q

A positive Ab titer is when there is a ___ -fold ___ in titer levels

A

4; rise

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13
Q

A change in titer is called a ___.

A

Seroconversion

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14
Q

High titer indicates?

A

Active infection

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15
Q

Low titer indicates?

A

Previous exposure

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16
Q

Molecular diagnostic tests analyze ___ or ___.

A

DNA or RNA

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17
Q

What are 3 types of molecular diagnostic tests?

A

PCR, Reverse Transcriptase PCR, Real-Time PCR

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18
Q

A positive ID allergy test shows what reaction?

A

Raised welt

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19
Q

A Tb skin test is testing for which microorganism?

A

Mycobacterium spp.

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20
Q

In FeLV what is the time from infection until presence of detectable Ag?

A

Within 28 days

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21
Q

Is FeLV an Ab or Ag test?

A

Ag

22
Q

In FeLV will the vaccine cause the test to be positive?

A

No

23
Q

What is FeLV confirmation test?

A

IFA

24
Q

In FIV what is the time from infection until presence of detectable Ab?

A

Within 60 days

25
Q

Is FIV an Ab or Ag test?

A

Ab

26
Q

Will FIV’s vaccine cause the test to be positive?

A

Yes

27
Q

What is FIV’s confirmation test?

A

Western Blot

28
Q

In FIV when should positive kittens be retested?

A

6 months old

29
Q

In FIV why can there be false positives?

A

Maternal derived Ab’s

30
Q

In Parvo how long does fecal shedding occur? How long after exposure?

A

7-10 days; 3-5 days

31
Q

Is Parvo an Ab or Ag test?

A

Ag

32
Q

Is Lyme Disease an Ag or Ab test?

A

Ab

33
Q

Detectable levels in Lyme Disease may not be present for how long post exposure?

A

4-6 weeks

34
Q

Will Lyme Disease’s vaccine cause a positive result?

A

No

35
Q

Is Ehrlichia an Ag or Ab test?

A

Ab

36
Q

Ehrlichia can also be diagnosed by?

A

Modulation in WBC’s

37
Q

Brucellosis is what type of test?

A

Rapid Slide Agglutination Test

38
Q

Is Brucellosis an Ag or Ab test?

A

Ab

39
Q

Is Heartworm in dogs an Ag or Ab test?

A

Ag

40
Q

When is heartworm in dogs first detectable?

A

5 months post infection

41
Q

How long after treatment in heartworm in dogs should Ag be undetectable?

A

4-5 months

42
Q

Heartworm-cat (Ab)

As early as ___ post-infection

A

2 months

43
Q

Heartworm-cat (Ag)

Only picks up Ag to which gender of worms?

A

Female

44
Q

Heartworm-cat

What is the best diagnostic method?

A

Echocardiogram

45
Q

Canine pregnancy- Relaxin

Produced by?

A

Placenta

46
Q

Canine pregnancy- Relaxin

How long following fertilization?

A

22-25 days

47
Q

Canine pregnancy- Relaxin

Levels peak around?

A

40-50 days

48
Q

Canine pregnancy- Relaxin

False negatives?

A

Small litters, one of more dead puppies

49
Q

Canine ovulation- Luteinizing Hormone

Bitch is fertile ___ following the LH surge

A

4-7 days

50
Q

Canine ovulation- Luteinizing Hormone

How often is the test ran?

A

Daily

51
Q

Canine ovulation- Progesterone

How often is the test ran?

A

Every 2-3 days

52
Q

Why is the foal/calf Ig test ran?

A

Useful in preventing disease related to failure of passive transfer of IgG from colostrum

Clin Lab III (1 decks)

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