Serology

Question 1

Sensitivity measures?

Answer 1

False negatives

Question 2

Specificity measures?

Answer 2

False positives

Question 3

Most serologic tests require a sample of?

Answer 3

Serum or plasma

Question 4

ELISA?

Answer 4

Enzyme linked immunosorbent assay

Question 5

ELISA test examples:

Answer 5

Heartworm, FeLV, Parvo

Question 6

CELISA?

Answer 6

Competitive enzyme linked immunosorbent assay

Question 7

Positive Latex Agglutination tests look like?

Answer 7

Agglutination

Question 8

RIM?

Answer 8

Rapid Immunomigration

Question 9

RIM positive test looks like?

Answer 9

2 areas of color, test, & control

Question 10

What does a Coomb’s test evaluate?

Answer 10

Autoantibodies

Question 11

A positive immunodiffusion test looks like?

Answer 11

A band is formed

Question 12

What indicates a positive IFA test?

Answer 12

Agglutination

Question 13

What two times are samples collected for ab titers?

Answer 13

Acute and convolscent

Question 14

A change in titer is called?

Answer 14

Seroconversion

Question 15

A positive Ab otter is when there is a _____ fold _____ in titer levels.

Answer 15

4, rise

Question 16

High titer?

Answer 16

Active infection

Question 17

Low titer

Answer 17

Previous infection

Question 18

Molecular diagnostic tests analyze?

Answer 18

DNA or RNA

Question 19

3 types of molecular diagnostic tests?

Answer 19

PCR, Reverse Transcriptase PCR, Real Time PCR

Question 20

A positive ID allergy test shows what reaction?

Answer 20

Raised welt

Question 21

A Tb skin test is testing for which microorganism?

Answer 21

Mycobacterium sp.

Question 22

FeLV- what is the time for infection until presence of detectable Ag?

Answer 22

28 days

Question 23

FeLV- Ab or Ag?

Answer 23

Ag

Question 24

FeLV- vx cause false positive?

Answer 24

No

Question 25

FeLV- what is the confirmation test?

Answer 25

IFA

Question 26

FIV- what is the time from infection until presence of detectable Ab?

Answer 26

60 days

Question 27

FIV- Ab or Ag?

Answer 27

Ab

Question 28

FIV- vx cause a false positive?

Answer 28

Yes

Question 29

FIV- confirmation test?

Answer 29

Western Blot

Question 30

FIV- when should positive kittens be retested?

Answer 30

6 mo

Question 31

FIV- why can there be false positives?

Answer 31

Kittens born to vx queens, MDA

Question 32

Parvo- how long does fecal shedding occur?

Answer 32

7-10 days

Question 33

Parvo- how long after exposure?

Answer 33

3-5 days

Question 34

Parvo- Ab or Ag?

Answer 34

Ag

Question 35

Lyme Dz- Ag or Ab test?

Answer 35

Ab

Question 36

Lyme Dz- detectable levels may not be present for how long after exposure?

Answer 36

4-6 weeks

Question 37

Lyme Dz- vx cause false positive?

Answer 37

No

Question 38

Ehrlichia- Ag or Ab?

Answer 38

Ab

Question 39

Ehrlichia- can also be Dx by?

Answer 39

Morale in WBC

Question 40

Brucellosis- type of test?

Answer 40

Rapid Slide Agglutination Test

Question 41

Brucellosis- Ag or Ab?

Answer 41

Ab

Question 42

Heartworm dogs- Ag or Ab?

Answer 42

Ag

Question 43

Heartworm dogs- first detectable?

Answer 43

5 mo post infection

Question 44

Heartworm dogs- how long after tx should Ag be undetectable?

Answer 44

4-5 mo

Question 45

Heartworm cats- Ab- as early as ___ mo post infection?

Answer 45

2

Question 46

Heartworm cats- Ag- only picks up which gender of worms?

Answer 46

Female

Question 47

Heartworm cats- best dx method?

Answer 47

Echocardiogram

Question 48

Canine pregnancy relaxin- produced by?

Answer 48

Placenta

Question 49

Canine pregnancy relaxin- how long following fertilization?

Answer 49

22-25 days

Question 50

Canine pregnancy relaxin- levels peak around?

Answer 50

40-50 days

Question 51

Canine pregnancy relaxin- false negatives?

Answer 51

Small litters, 1 or more dead puppies

Question 52

Canine ovulation LH- bitch is fertile _____ days after LH surge?

Answer 52

4-7

Question 53

Canine ovulation LH- how often is the test ran?

Answer 53

Daily

Question 54

Canine ovulation progesterone- how often is the test ran?

Answer 54

Every 2-3 days

Question 55

Foal/calf Ig- why is this s test ran?

Answer 55

Prevent dz related to failure of passive transfer of IgG from colostrum