Serology Flashcards

1
Q

Sensitivity measures?

A

False negatives

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2
Q

Specificity measures?

A

False positives

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3
Q

Most serologic tests require a sample of?

A

Serum or plasma

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4
Q

ELISA?

A

Enzyme linked immunosorbent assay

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5
Q

ELISA test examples:

A

Heartworm, FeLV, Parvo

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6
Q

CELISA?

A

Competitive enzyme linked immunosorbent assay

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7
Q

Positive Latex Agglutination tests look like?

A

Agglutination

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8
Q

RIM?

A

Rapid Immunomigration

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9
Q

RIM positive test looks like?

A

2 areas of color, test, & control

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10
Q

What does a Coomb’s test evaluate?

A

Autoantibodies

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11
Q

A positive immunodiffusion test looks like?

A

A band is formed

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12
Q

What indicates a positive IFA test?

A

Agglutination

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13
Q

What two times are samples collected for ab titers?

A

Acute and convolscent

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14
Q

A change in titer is called?

A

Seroconversion

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15
Q

A positive Ab otter is when there is a _____ fold _____ in titer levels.

A

4, rise

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16
Q

High titer?

A

Active infection

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17
Q

Low titer

A

Previous infection

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18
Q

Molecular diagnostic tests analyze?

A

DNA or RNA

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19
Q

3 types of molecular diagnostic tests?

A

PCR, Reverse Transcriptase PCR, Real Time PCR

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20
Q

A positive ID allergy test shows what reaction?

A

Raised welt

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21
Q

A Tb skin test is testing for which microorganism?

A

Mycobacterium sp.

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22
Q

FeLV- what is the time for infection until presence of detectable Ag?

A

28 days

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23
Q

FeLV- Ab or Ag?

A

Ag

24
Q

FeLV- vx cause false positive?

A

No

25
Q

FeLV- what is the confirmation test?

A

IFA

26
Q

FIV- what is the time from infection until presence of detectable Ab?

A

60 days

27
Q

FIV- Ab or Ag?

A

Ab

28
Q

FIV- vx cause a false positive?

A

Yes

29
Q

FIV- confirmation test?

A

Western Blot

30
Q

FIV- when should positive kittens be retested?

A

6 mo

31
Q

FIV- why can there be false positives?

A

Kittens born to vx queens, MDA

32
Q

Parvo- how long does fecal shedding occur?

A

7-10 days

33
Q

Parvo- how long after exposure?

A

3-5 days

34
Q

Parvo- Ab or Ag?

A

Ag

35
Q

Lyme Dz- Ag or Ab test?

A

Ab

36
Q

Lyme Dz- detectable levels may not be present for how long after exposure?

A

4-6 weeks

37
Q

Lyme Dz- vx cause false positive?

A

No

38
Q

Ehrlichia- Ag or Ab?

A

Ab

39
Q

Ehrlichia- can also be Dx by?

A

Morale in WBC

40
Q

Brucellosis- type of test?

A

Rapid Slide Agglutination Test

41
Q

Brucellosis- Ag or Ab?

A

Ab

42
Q

Heartworm dogs- Ag or Ab?

A

Ag

43
Q

Heartworm dogs- first detectable?

A

5 mo post infection

44
Q

Heartworm dogs- how long after tx should Ag be undetectable?

A

4-5 mo

45
Q

Heartworm cats-

Ab- as early as ___ mo post infection?

A

2

46
Q

Heartworm cats-

Ag- only picks up which gender of worms?

A

Female

47
Q

Heartworm cats- best dx method?

A

Echocardiogram

48
Q

Canine pregnancy relaxin- produced by?

A

Placenta

49
Q

Canine pregnancy relaxin- how long following fertilization?

A

22-25 days

50
Q

Canine pregnancy relaxin- levels peak around?

A

40-50 days

51
Q

Canine pregnancy relaxin- false negatives?

A

Small litters, 1 or more dead puppies

52
Q

Canine ovulation LH- bitch is fertile _____ days after LH surge?

A

4-7

53
Q

Canine ovulation LH- how often is the test ran?

A

Daily

54
Q

Canine ovulation progesterone- how often is the test ran?

A

Every 2-3 days

55
Q

Foal/calf Ig- why is this s test ran?

A

Prevent dz related to failure of passive transfer of IgG from colostrum