serology Flashcards
direct fluorescent
a known ab combined with fluorescent tag added to unknown ag
pos will turn green
ELISA
hiv
comp. enzyme-labeled competes with patient ag for ab spots
non competitive- higher sensitivity
complement fixation
if lysis occurs in stage 2, complement didnt fix during stage 1 so negative test
RPR
does not require inactivation of plasma/serum
davidson test
a positive rxc is indicated by complete removal of anti-sheep agglutinins (abs) by guinea pig kidneys and/or sheep RBC
ANA test
SLE
causative agent of syphillis
treponema pallidum
causative agent of mono
epstein barr virus
what ab is produced after first exposure
igm
what ab is produced in greatest concentration
igg
what ab is greatest concentration after 2nd exposure
igg
what ab is associated with allergic reactions
ige
davidson
absorbed by guinea pig kidney
reagin
ab formed against lipid matieral from damaged cells (syphillis)
prozone
antibody excess so failure of serological rxc
cross reaction
when an ag is so structurally similiar to a second antigen that it will react with ab to the second ag
FC
activates complement
passive acquired immunity
mothers milk
active acquired immunity
natural exposure or vaccine
adoptive immunity
immune donor
biological false positive
non-treponemal test detedcts ab in serum that can be caused by agents other than syphilis
what ig class can bind complement
igg
igm
rubella vaccination
active acquired immunity
hep B
HbsAg HBeAg Igm anti-HBc anti-HBe anti-HBs
what are the other modes of transmission for Hep
fecal oral
blood borne
test most widely used to detect HIV ab
ELISA
how long on average does it take an individual to show HIV ab affter exposure
6-12 weeks
RAST
serological determination of antigen-specific IGe
allergic rxc
multiple myeloma
igG
define window phase in HIV testing
the length of time after infection that it takes for the virus to become detectable by HIV tests
