serology Flashcards

1
Q

direct fluorescent

A

a known ab combined with fluorescent tag added to unknown ag

pos will turn green

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2
Q

ELISA

A

hiv
comp. enzyme-labeled competes with patient ag for ab spots

non competitive- higher sensitivity

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3
Q

complement fixation

A

if lysis occurs in stage 2, complement didnt fix during stage 1 so negative test

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4
Q

RPR

A

does not require inactivation of plasma/serum

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5
Q

davidson test

A

a positive rxc is indicated by complete removal of anti-sheep agglutinins (abs) by guinea pig kidneys and/or sheep RBC

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6
Q

ANA test

A

SLE

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7
Q

causative agent of syphillis

A

treponema pallidum

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8
Q

causative agent of mono

A

epstein barr virus

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9
Q

what ab is produced after first exposure

A

igm

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10
Q

what ab is produced in greatest concentration

A

igg

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11
Q

what ab is greatest concentration after 2nd exposure

A

igg

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12
Q

what ab is associated with allergic reactions

A

ige

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13
Q

davidson

A

absorbed by guinea pig kidney

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14
Q

reagin

A

ab formed against lipid matieral from damaged cells (syphillis)

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15
Q

prozone

A

antibody excess so failure of serological rxc

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16
Q

cross reaction

A

when an ag is so structurally similiar to a second antigen that it will react with ab to the second ag

17
Q

FC

A

activates complement

18
Q

passive acquired immunity

A

mothers milk

19
Q

active acquired immunity

A

natural exposure or vaccine

20
Q

adoptive immunity

A

immune donor

21
Q

biological false positive

A

non-treponemal test detedcts ab in serum that can be caused by agents other than syphilis

22
Q

what ig class can bind complement

A

igg

igm

23
Q

rubella vaccination

A

active acquired immunity

24
Q

hep B

A
HbsAg
HBeAg
Igm anti-HBc
anti-HBe
anti-HBs
25
Q

what are the other modes of transmission for Hep

A

fecal oral

blood borne

26
Q

test most widely used to detect HIV ab

A

ELISA

27
Q

how long on average does it take an individual to show HIV ab affter exposure

A

6-12 weeks

28
Q

RAST

A

serological determination of antigen-specific IGe

allergic rxc

29
Q

multiple myeloma

A

igG

30
Q

define window phase in HIV testing

A

the length of time after infection that it takes for the virus to become detectable by HIV tests