serology Flashcards
direct fluorescent
a known ab combined with fluorescent tag added to unknown ag
pos will turn green
ELISA
hiv
comp. enzyme-labeled competes with patient ag for ab spots
non competitive- higher sensitivity
complement fixation
if lysis occurs in stage 2, complement didnt fix during stage 1 so negative test
RPR
does not require inactivation of plasma/serum
davidson test
a positive rxc is indicated by complete removal of anti-sheep agglutinins (abs) by guinea pig kidneys and/or sheep RBC
ANA test
SLE
causative agent of syphillis
treponema pallidum
causative agent of mono
epstein barr virus
what ab is produced after first exposure
igm
what ab is produced in greatest concentration
igg
what ab is greatest concentration after 2nd exposure
igg
what ab is associated with allergic reactions
ige
davidson
absorbed by guinea pig kidney
reagin
ab formed against lipid matieral from damaged cells (syphillis)
prozone
antibody excess so failure of serological rxc