Sequencing by Synthesis Flashcards
1
Q
Describe library preparation (QC)
A
2
Q
Calculate the amount of library required instrument lo ding
A
3
Q
Describe a flow cell and cluster
A
4
Q
Describe traditional cluster generation
A
5
Q
Describe pair-end sequencing
A
6
Q
Describe Illumina sequencing by synthesis
A
7
Q
Define a Q score
A
8
Q
Calculate: Library concentration
A
nM = (Dye neg/uL x 10^6) / (660 x library size bp)
