Sequencing

Question 1

Sanger sequencing

Answer 1

Dideoxy nucleotides terminate DNA strand elongation, which allows determination of the DNA sequence.

Question 2

Key components of sanger sequencing

Answer 2

DNA polymerase, DNA template, primers, deoxynucleotide triphosphates (dNTPs) and small amount of dideoxynucleotide triphosphates.

Question 3

Human Genome project

Answer 3

13 year long, 20 universities, worldwide, 3 billion dollar science project.

Question 4

Next Generation sequencing

Answer 4

A collection of modern sequencing technologies that allow rapid sequencing.

Question 5

Roche 454 pyrosequencing

Answer 5

Uses beads to clonally amplify and sequencing through the use of pyrosequences.

Question 6

Illumina Sequencing steps

Answer 6

Library preparation
Cluster amplification
Sequencing process

Question 7

3rd generation sequencing

Answer 7

Capable of sequencing a single molecule without a need for amplification.

Question 8

PacBio

Answer 8

Long reading sequencing with high error rates and costs, but invaluable for hybrid DNA assemblies.

Question 9

Fluorescent labelling in sequencing

Answer 9

Fluorescent labels in sequencing technologies are expected to further drive down costs and enhance capabilities.