seperation techniques 1 Flashcards
1
Q
why do you need to prepare before seperation?
A
- prepare substance to study or for commercial use
2
Q
what is determination?
A
- qualifying a substance in a biological sample. includes qualification and quantification
3
Q
what is separation with dialysis
A
- based on commercial membranes with pores of pre- determined size
- unit = molecular weight cut off
- removes all molecules/ ions = so good for desalting
but SLOW AND LABOROUS
4
Q
what are dialysis membranes made of?
A
- cellophane
- cellulose nitrate
- various plastics
5
Q
what is filtrate and retentate?
A
filtrate = what goes through
retentate = what stays behind
6
Q
CONDITIONS FOR ROLL OF VISKING TUBING?
A
must be hydrated and pre- treated before use to remove contaminants
7
Q
what is ultrafiltration?
A
- similar membranes are filled with protein solutions and then a force is applied to force the proteins and water through the membrane.
8
Q
what is central ultrafiltration?
A
- uses centrifuge to apply force to the solution - pulling it through
9
Q
in centrifuge what is pulled through?
A
- water, salts, and everything below MWCO
10
Q
what is pressure ultrafiltration?
A
- same as centrifuge
- uses chamber where base is filter and top connected to cylinder of argon gas
- gas pressure above the liquid pushes it throught the filter
11
Q
what is chromotography?
A
- useful fro any type of biological molecule
- can be used to seperate on the basis of shape, size, ionisation, polarity, hydrophobicity
12
Q
what is chromatography theory
A
- solution of solutes dissolved in solvent
13
Q
what is stationary phase?
A
eg. paper, silica gel, waxy beads, plastic beads
14
Q
what is mobile phase?
A
- buffer solutions, helium, nitrogen, organic solvents
15
Q
chromatography paper
A
- paper is cheaper,
- but slower
