Separation techniques Flashcards
1
Q
Centrifugation
A
Separates a solution on density (creating a pellet and supernatant)
2
Q
Paper chromatography
A
Separates a solution on solubility
3
Q
Affinity chromatography
A
A gel column with specific molecules is formed; target proteins in the solution bind to the gel, whereas non-target proteins are washed away.
4
Q
Gel electrophoresis
A
Current flowing through a buffer to separate charged proteins
5
Q
Native gels
A
Separates proteins on size, shape, and charge
6
Q
SDS-PAGE
A
Denaturing proteins, giving them all equally negative charges, meaning they’ll be separated on size alone
7
Q
Iso-electric point
A
The pH at which a specific protein is soluble and therefore precipitates out
