Seminar content Flashcards
A new compound (called KMTI223a) has been identified which acts as a specific inhibitor
of Suvar39. If cells are treated with KMTI223a which of the following scenarios is the most
likely?
a. Increased levels of histone H3 lysine 9 methylation
b. Increased levels of histone H3 lysine 36 methylation
c. Decreased levels of histone H3 lysine 9 methylation
d. Decreased levels of histone H3 lysine 36 methylation
e. No change in levels of histone H3 lysine 36 methylation or lysine 9 methylation
c
Class 3 HDACs including the Sir2 family or ‘Sirtuins’ are dependent on which molecule?
NAD
If an osteosarcoma cell is exposed to UV irradiation what are the most likely effects on the level of p53 and its phosphorylation at serine 15?
p53 levels would INCREASE
phosphorylation would also INCREASE
a compound has been identified that causes the activation of the TNF alpha receptor. what effect would you expect this compound to have on IkB?
it would result in the proteolytic degradation of IkB
which statement(s) about eukaryotic RNA polymerase II is/are CORRECT?
Select one or more alternatives:
List
a. It is responsible for the transcription of miRNA
b. It is responsible for the transcription of tRNA
c. Some of its subunits are homologous to those of bacterial RNA polymerase
d. It is located in the nucleolus
e. The largest subunit of has a series of repeats known as the carboxy terminal domain
(CTD)
a, c, e
Which statement(s) about the spliceosome is/are CORRECT?
Select one or more alternatives:
List
a. It catalyses the removal of introns from pre-mRNA
b. Its function requires ATP
c. It is a small complex containing <20 proteins
d. It contains snRNAs that are responsible for the catalytic steps in splicing
e. It contains exonucleases required for the 3’ to 5’ degradation of mRNA
a, b, d
an RNA editing event that occurs in glutamate receptor mRNAs in
neurons. What enzyme catalyses this reaction?
ADAR2
PKR is a kinase that is activated by double-stranded RNA (viral infection). What
important regulator of translation does it phosphorylate?
eIF2 alpha
What
influence would you expect low iron levels to have on 5’ UTR human mRNA?
a. increase the stability of the mRNA
b. decrease the stability of the mRNA
c. decrease splicing of the mRNA
d. increase translation of the mRNA
e. idecrease translation of the mRNA
d - decrease translation of the mRNA
Which statement(s) about the m7GpppN 5’ cap is/are CORRECT?
a. it protects mRNA from degradation by 5’-3’ nucleases
b. it protects mRNA from degradation by 3’-5’ nucleases
c. it facilitates splicing
d. it facilitates export from the nucleus
e. it is critical for translation of most mRNAs
a, c, d, e
Which of the following statements about pre-mRNA splicing are CORRECT?
a. it causes a major change in the composition of the mRNA relative to the genomic
sequence
b. Almost all yeast genes have multiple introns
c. Splicing is protein catalysed event
d. Alternative splicing is key to providing multiple protein isoforms from one gene
e. Defects in mRNA splicing are linked to multiple genetic diseases
a, d, e
which was the first nuclear histone acetyl transferase to be identified?
Gcn5
which complex is the main 3’ to 5’ exonuclease activity in a cell that is involved in RNA turnover and processing and has multiple nuclease activities?
exosome
what is the function of p14ARF?
a. To bind to p53 and inhibit its DNA-binding
b. To bind to Mdm2 and activate p53
c. To stabilise the interaction of Mdm2 with p53
d. To phosphorylate p53
e. To proteolytically degrade p53
b - to bind to Mdm2 and activate p53
Concerning translation in eukaryotes: What protein interacts with both the 3’ end of the
mRNA and with eIF4G to mediate mRNA circularisation?
Poly A binding protein (PABP)
