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MTAP hematology Part 2 > Seminar > Flashcards

Seminar Flashcards

1
Q

Also known as Coulter principle

A

Electronic impedance

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2
Q

Electronic impedance is based on the detection and measurement of changes in electrical resistance produced by cells as they traverse a large aperture

T or F

A

F

Small aperture

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3
Q

Electronic impedence is electrical resistance between the two electrodes, or impedance in the current, occurs as the cells pass through the sensing aperture, causing voltage pulses that are measurable

T or F

A

T

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4
Q

In electronic impedance:

The number of pulses is _____________ to the numbers of cells counted

A

proportional

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5
Q

In electronic impedance:

The height of the voltage pulse is ___________ proportional to cell volume.

A

directly

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6
Q

In electronic impedance:

Blood sample is distributed into two channels: ____/PLT and WBC/____

A

RBC

HGB

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7
Q

In electronic impedance:

RBC / Platelet Channel:
The machine determines the component if cell volumes are:
− 36-360 fL = ____
− 2-20 fL = _____

A

RBCs

Platelets

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8
Q

In electronic impedance:

WBC / Hemlglobin Channe

Adds lysing agent to remove RBCs

T or F

A

T

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9
Q

In electronic impedance:

WBC / Hemlglobin Channe

3-part WBC differential count are:
− 35-90 fL = ______________
− 91-160 fL = _____________
− 161-450 fL = ______________

A

Lymphocytes

Monocytes

Granulocyte

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10
Q

When the RBCs are lysed, the hemoglobin comes out.
− The standard method for Hgb measurement is the ______________________________ measured
spectrophotometrically at 540 nm

A

Cyanmethemoglobin method (HiCN),

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11
Q

In RBC histogram:

The x-axis represents the cell volume.

The y-axis represents the relative number or frequency

T or F

A

T

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12
Q

NOTE:

RBC histogram is basically MCV that is being plotted in a graph

A
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13
Q

Usually used in conjunction with electronic impedance

A

Radiofrequency

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14
Q

RF + EI is now capable of _-part differential count

A

5

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15
Q

The 5 part differential count includes?

A

neutrophil, eosinophil, basophil,
monocyte, and lymphocyte

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16
Q

3-part differential count includes?

A

lymphocyte, monocyte, and granulocytes

17
Q

The cell interior density is proportional to pulse height or change in the RF signal

T or F

A

T

18
Q

Conductivity is determined using a low frequency electromagnetic probe that provides information on the cell internal constituents.

T or F

A

F

High

19
Q

a principle that is the detection of scattered rays and their conversion into electrical signals is accomplished by photodetectors at specific angles

A

OPTICAL LIGHT SCATTER

20
Q

______________ light scatter (__degrees) correlates with cell volume.

____ light scatter (___degrees) correlates with degree of internal complexity

A

Forward angle, 0

Side, 90

21
Q

Other name of optical light scatter?

A

Laser based technology

22
Q

Laser based technology is capable of 3 part differential count

T or F

A

F

5

23
Q

Error in automation:

Cold agglutinins may affect what parameter due to clumping at low temp?

_______ RBC
_______ MCHC
_______ MCV

A

Decrease

Increase

Increase

24
Q

Error in automation:

Lipemia, icteric sample may affect what parameter ?

_______ Hgb
_______ MCHC

A

All increase

25
Q

Error in automation:

Lyse resistant RBCs with abnormal Hb

_______ Hgb
_______ WBC

A

All increase

26
Q

Error in automation:

Microcytes or schistocytes may affect what parameter ?

_______ PLT
_______ RBC

A

Increase

Decrease

27
Q

Lipemia causes high TGL while icteric sample causes high BIL which causes turbidity that affects the hemoglobin which is measures with spectrophotometric at 540nm

T or F

A

T

28
Q

Lyse resistant RBC with abnormal hemoglobin are sickle cells (lyse resistant) with hemoglobin S (causes turbidity). Due to this RBCs that are resistant to lysing agent, the automation recognizes them as WBCs and not RBCs which results in falsely increased WBC and Hemoglobin.

T or F

A

T

29
Q

Schistocytes are fragmented RBCs, the machine recognizes them as platelets due to their small sizes which results in falsely decrease RBC result while the platelets are false increased

T or F

A

T

30
Q

Nucleated RBCs and Megakaryocyte fragments are both lyse resistant which results in the machine recognizing them as WBC.

T or F

A

T

31
Q

Platelets clumps will be recognized as WBC by the machine which causes falsely decreased platelets and falsely increased WBC

T or F

A

T

32
Q

WBC count more than 100,000/ul will cause turbidity which automatically result in falsely increase hemoglobin. RBC will also be falsely increased

T or F

A

T

33
Q

Old specimen causes RBC swelling, platelet swelling, and plt disintegration

T or F

A

T

MTAP hematology Part 2 (6 decks)

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