Seminar Flashcards
Also known as Coulter principle
Electronic impedance
Electronic impedance is based on the detection and measurement of changes in electrical resistance produced by cells as they traverse a large aperture
T or F
F
Small aperture
Electronic impedence is electrical resistance between the two electrodes, or impedance in the current, occurs as the cells pass through the sensing aperture, causing voltage pulses that are measurable
T or F
T
In electronic impedance:
The number of pulses is _____________ to the numbers of cells counted
proportional
In electronic impedance:
The height of the voltage pulse is ___________ proportional to cell volume.
directly
In electronic impedance:
Blood sample is distributed into two channels: ____/PLT and WBC/____
RBC
HGB
In electronic impedance:
RBC / Platelet Channel:
The machine determines the component if cell volumes are:
− 36-360 fL = ____
− 2-20 fL = _____
RBCs
Platelets
In electronic impedance:
WBC / Hemlglobin Channe
Adds lysing agent to remove RBCs
T or F
T
In electronic impedance:
WBC / Hemlglobin Channe
3-part WBC differential count are:
− 35-90 fL = ______________
− 91-160 fL = _____________
− 161-450 fL = ______________
Lymphocytes
Monocytes
Granulocyte
When the RBCs are lysed, the hemoglobin comes out.
− The standard method for Hgb measurement is the ______________________________ measured
spectrophotometrically at 540 nm
Cyanmethemoglobin method (HiCN),
In RBC histogram:
The x-axis represents the cell volume.
The y-axis represents the relative number or frequency
T or F
T
NOTE:
RBC histogram is basically MCV that is being plotted in a graph
Usually used in conjunction with electronic impedance
Radiofrequency
RF + EI is now capable of _-part differential count
5
The 5 part differential count includes?
neutrophil, eosinophil, basophil,
monocyte, and lymphocyte
3-part differential count includes?
lymphocyte, monocyte, and granulocytes
The cell interior density is proportional to pulse height or change in the RF signal
T or F
T
Conductivity is determined using a low frequency electromagnetic probe that provides information on the cell internal constituents.
T or F
F
High
a principle that is the detection of scattered rays and their conversion into electrical signals is accomplished by photodetectors at specific angles
OPTICAL LIGHT SCATTER
______________ light scatter (__degrees) correlates with cell volume.
____ light scatter (___degrees) correlates with degree of internal complexity
Forward angle, 0
Side, 90
Other name of optical light scatter?
Laser based technology
Laser based technology is capable of 3 part differential count
T or F
F
5
Error in automation:
Cold agglutinins may affect what parameter due to clumping at low temp?
_______ RBC
_______ MCHC
_______ MCV
Decrease
Increase
Increase
Error in automation:
Lipemia, icteric sample may affect what parameter ?
_______ Hgb
_______ MCHC
All increase
Error in automation:
Lyse resistant RBCs with abnormal Hb
_______ Hgb
_______ WBC
All increase
Error in automation:
Microcytes or schistocytes may affect what parameter ?
_______ PLT
_______ RBC
Increase
Decrease
Lipemia causes high TGL while icteric sample causes high BIL which causes turbidity that affects the hemoglobin which is measures with spectrophotometric at 540nm
T or F
T
Lyse resistant RBC with abnormal hemoglobin are sickle cells (lyse resistant) with hemoglobin S (causes turbidity). Due to this RBCs that are resistant to lysing agent, the automation recognizes them as WBCs and not RBCs which results in falsely increased WBC and Hemoglobin.
T or F
T
Schistocytes are fragmented RBCs, the machine recognizes them as platelets due to their small sizes which results in falsely decrease RBC result while the platelets are false increased
T or F
T
Nucleated RBCs and Megakaryocyte fragments are both lyse resistant which results in the machine recognizing them as WBC.
T or F
T
Platelets clumps will be recognized as WBC by the machine which causes falsely decreased platelets and falsely increased WBC
T or F
T
WBC count more than 100,000/ul will cause turbidity which automatically result in falsely increase hemoglobin. RBC will also be falsely increased
T or F
T
Old specimen causes RBC swelling, platelet swelling, and plt disintegration
T or F
T
