Semi Finals Flashcards
Luminometer lacks in
Light source
Chemiluminiscence is measured through
Luminometer
In indirect IFA what is the composition of the reaction
Solid phase ab + ab from sample + fluoroscenated anti Ig
Name 4 fluorochromes
Fluorescein thioisocyanate
Phycocyanin
Texas red
Tetramethyl rhodamine
Adavantage of indirect IFA
Higher sensitivity
In direct IFA, what viral agent that is positive for cell surface antigen
Chlamydia
Disadvantage of indirect IFA
Queching (lowered flouroscence)
EMIT stands for
Enzyme multiplied immunoassay technique
3 viruses being detected in Direct IFA
HSV, EBV, CMV
It uses fluoroscent compound
Fluorescence immunoaasay
Developed EMIT
Syva corporation
This utilizes membrane bound cassetes
Rapid immunoassay
Labels being used in fluorecence immunoassay
Fluorophores or fluorochromes
Assay that Detects patient’s antibody
Capture assay
Purpose of washing?
To remove unbound antibody
Principle of pregnancy testing
Immunochromatography
Instrument used in direct IFA
Cell flow cytometry
A heterogenous enzyme immunoassay that is also known as Indirect ELISA
Non competitive heterogenous enzyme immunoassay
Enzymes used in EIA
HPO, ALP, glucose oxidase
It requires washing to remove unbound antibody
Heterogenous immunoassay
Advantage of enzymes when being utillized in EIA
Highly stable
Extreme specifity
Cant be altered by inhibitors
Immunassay that uses radioactive substances
Radioimmunoassay
Most common enzyme used in EIA
HPO
Type of immunoassay that reagents are simultaenously being added
Competitive immunoaasay
It uses enzymes as labels
Enzyme immunoassay
2 radioactive substances measured at the beta counter
14 carbon
3 hydrogen
Sandwhiched antigen by antibody
Capture assay
In vivo sensitization
DAT
Instrument used in radioimmunoassay
Scintillation counter
Used to check results in IAT
Check cells
It uses labels to detect serological reactions
Labelled immunoassay
2 radioactive substance that is measure at the gamma counter
131 Iodine
125 Iodine
Postive result in agglutination inhibition
No agglutination
Examples of DAT
HDN
HTR
AIHA
Type of immunoassay that competes for binding site
Competitive immunoaasay
The carrier in coagglutination
Bacteria
Requirement for IAT?
Incubation
In vitro sensitization
IAT
Region of HCG detected in pregnancy testing
Beta region
In reverse agglutination what is being detected
Antibody
Relationship in non competitive immunoassay
Direct proportional
AKA coombs test
Anti human globulin test
In coagglutination the FC binds to what part of S. Aureus
Protein A
It uses bacteria as the carrier
Coagglutination
Antigen are artificially attached to the carrier
Passive agglutination
What is being detected in passive agglutination
Antigens
Antigen are found naturally found on the surface of the particles
Direct agglutination
Widal test is used to detect what type of pathologic condition
Thypoid fever
Test performed to detect rickettsia infection
Weil felix
Numerous clumps
2+
Antibodies are attached to particulate carriers
Reverse agglutination
Other name of coombs cells
Check cells
It promotes the process of agglutination
Enchancement media
Used to ddetect mycoplasma pneumoniae infection
Cold agglutination
Biggest Immunoglobulin
IgM
Uses of IAT?
Cross matching
Ab Identification
Ab screen
Principle of blood typing
Red cell hemeagglutination
One solid clump
4+
Provides positive ion
Albumin 5-30%
Questionable result in agglutination
1+
Negative result in agglutination inhibition
Agglutination
Used to serotype salmonella spp.
Kauffmanand white scheme
In agglutination reaction, antigen involved are
Particulate
Transplacental immunoglobulin
IgG
It destroys the sialic acid
Enzymes
It is the initial combination of antigen and antibody
Sensitization
Term used to describe the distance of RBC from one another
Zeta potential
Useful procedures for the identification of monoclonal gamopathy
Immunoelectrophoresis
It provides the negative charge of an RBC
Sialic acid
In agglutination reactions antibody involved are
Agglutinous
Positive result in immunofixation electrophoresis
Precipitin bonds
Invented immunoelectrophoresis
Gravar and williams
Compostition of immunofixation electrophoresis
Immunoprecipitation + electrophoresis
End result in rocket immunoelectrophoresis
Conical shaped precipitin line
Several large clump
3+
Absent canals in immunofixation electrophoresis
Through
Process where the formation of aggregates starts
Lattice phase
Double diffusion, double dimension is also known as
Ouchterlony technique
Used to quantitate immunoglobulins
Rocket electrophoreis
Used to speed things up in electrophoretic procedures
Electric current
Technique wherein both antibody and antigen are moving
Ouchterlony technique
This technique is a first generation test like in HbsAg testing
Ouchterlony testing
Other name of mancini method
End point diffusion
Technique ised to separate molecules using electrical current
Electrophoresis
Other name of Fahey and McKelvey method
Kinetic diffusion
Interpretation of a smooth curve in ouchterlony testing
Identity (complete)
Rocket electrophoresis is a combination of electrophoresis and
RID
Spur formation means
Partial identity
Cancer cells that invades the tissue
Malignant
Intersection formation means
No identity
In oudin test the antibody is incorporated in
Agarose gel
Square of diameter is proportion to the concentration of ag-ab reaction
Mancini method
The end result in radial immunodiffusion
Precipitin ring
Oudin test is AKA
Single diffusion single dimension technique
No electrical current is used
Passive immunodiffusion
Insturment utilized in turbidimetric measurements
Spectrophotometer
Measure light that is scattered in a particular angle
Nephelometry
The optimum ration between antigen and antibody is termed as
Zone of equivalence
End result in oudin test
Precipitin line
Relationship of both turbidimetry and nephelometry
Direct proportional
Instrument used in nephelometry
Nephelometer
Excess in prozone
Antibody
Antigen and antibody are placed on the well dorectly opposite to each other
Counter immunoelectrophoresis
Examples of tertiary immunological reaction
Phagocytosis
Opsonizatio
Excess in postzone
Antigen
Removes water
PEG or dextran
Serum is stored in
Freezer
In precipitation reaction antigens involved are
Soluble
Result that is given in prozone and postzone conditions
False negative
Immunological reaction where it demonstrate antigen and antibody reaction
Secondary reactions
Immunological reaction where the combination of antigen and antibody complex is non visible
Primary immunological reaction
VDRL is used to detect
Syphilis
Chemical way of inactivating serum
Addition of choline chloride
Example of chemical inactivation of serum
Rapid plasma reagin
Used to detect unknown antigen using known antibody
Direct serological test or forward serological test
Red cell is stored in
Ref
Rapid plasma reagin is used to detect what kind of pathologic illness
Syphilis infection
Type of immunological reaction that is immunologically in vivo
Tertiary reaction
Setting for reinactivation of serum
56degrees celcius for 10mins
Test that involves antigen and antibody reaction
Serological test
Type of serologic test wherein known antigen is used to detect unknown antibody
Backward serological test or indirect serological test
Example of forward serological test
RC blood typing
Sugar present in Anti A antisera
N acetyl galactosamine
Sugar present in Anti B antisera
D galactose
Type of cancer treatment wherein antigen are given
Active
Capable of causing agglutination is what kind of immunoglobulin
IgM
Chemiluminiscence is composed of
Chemical reaction and luminal compound
Hormone present in pancreatic gastrinoma
Gastrin
Type of cancer treatment where antibody is given
Passive