Selection of T Cell Receptor Repertoire and CD4/CD8 Lineage Commitment Flashcards
How does the thymus allow for T cell progenitors to migrate into it (what does it do)?
Releases chemokines into the blood stream that reach the bone marrow so T cells will follow that back to the thymus.
What is the most frequent cells in the thymus?
Cortical/medullary epithelial cells (stromal cells)
What is Hassall’s corpuscle?
In the thymus medulla, is an aggregation of mature T lymphocytes (mostly T regulatory lymphocytes)
Which part of the thymus does late stage T cell development occur?
Medulla
What does CD stand for in T cell co-receptors?
Cluster of differentiation
Recap how flow cytometry can characterise T cell development
- Can show us if the cell is double negative, double positive or CD4 or CD8 positive
- Can also sub characterise double negative cells into DN1-DN4
How does the quantitiy of DN/DP/CD4/CD8 cells differ from the thymus in contrast to other organs such as the spleen?
- In the Thymus the most abundant type of T cell is the double negative (DN) but in other organs there will be very few DN and almost all cells will wither be CD4 or CD8
- as the selection to CD8 or CD4 happens in the thymus
Does T cell maturation and T cell receptor rearrangement occur sequentially or simulatenously?
Simultaneously
We know that T cells chose to be CD8 or CD4, but does a gamma delta T cell also need to make this choice??
The ‘normal’ T cell differentiation passage is for the alpha/beta T cells and the gamma/delta branch off before. Meaning that I think that gamma/delta do not express either CD4 or CD8
At what stage do γδ T cells branch off in the normal T cell development in the thymus?
Between DN2 and DN3
In which stage of human development are γδ more abundant than αβ?
During early foetal development
δγ T cells bearing specific receptors end up in the {?}, {?}, {?} and other places
δγ T cells bearing specific receptors end up in the skin, gut, uterus and other places
Describe the difference in the mechanism used to detect antigens by δγ T cells as opposed to conventional T cells
δγ T cells do NOT recognise peptides via MHC, but instead recognise them directly, similar to how an antibody would.
In some cases, ligands for δγ T cell TCRs are {?} that are upregulated under stress conditions
In some cases, ligands for δγ T cell TCRs are self proteins that are upregulated under stress conditions
In humans, circulating δγ cells recognise a {?(not peptide)} antigen in {?}. δγ cells also play a role in cancer surveillance.
In humans, circulating δγ cells recognise a phospholipid (not peptide) antigen in tuberculosis. δγ cells also play a role in cancer surveillance.